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  5. Invitrogen™ Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit

Invitrogen™ Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit

Numéro de catalogue. EIAANPX10
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10 x 96 Tests
96 Tests
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EIAANPX10 10 x 96 Tests
EIAANP 96 Tests
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Numéro de catalogue. EIAANPX10 Fournisseur Invitrogen™ Code fournisseur EIAANPX10
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Description

ELISA

The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma,urine or cell culture medium.

Principle of the method

The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma,urine,and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.

Spécifications

Assay Range 0.741 to 180 ng/mL
Assay Sensitivity 0.25 ng/mL
Description Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit
Assay Multiplex Assay on the Luminex Platform
Conjugate HRP
Detection Method Fluorescence
Product Type ELISA
Sample Type Plasma, Supernatant, Urine
Sufficient For 10 x 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 7.6%
Intraassay CV 6.7
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Plasma 50 μL, Supernatant 50 μL, Urine 12.5 μL
Storage Requirements -20°C
Target ANP
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 30 mins
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