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Applied Biosystems™ SYBR™ Green Universal Master Mix 
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Supplier: Applied Biosystems™ 4344463
Description
Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
- AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
- dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
- Proprietary buffer enhancements ensure performance and reliability
- Premixed components stored at 2° to 8°C significantly reduce assay setup time
Maximum Flexibility and Convenience:
- Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
- SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
- The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
- Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
- SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
- Store at -20°C
Specifications
| SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
|
| Concentrated | |
| High | |
| AmpliTaq Gold DNA Polymerase | |
| Fluorescence Intensity | |
| SYBR Green | |
| SYBR™ | |
| 1 x 1 mL | |
| 40 Reactions of 50 μL | |
| Gene Expression | |
| Built-In Hot Start | |
| DNA |
| SYBR | |
| Tube | |
| qPCR | |
| Standard | |
| 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700 | |
| ROX (Pre-mixed) | |
| Real Time PCR SYBR Master Mix | |
| Wet Ice | |
| Not Target-Specific | |
| 2X | |
| 40 Reactions | |
| 1 x 1 mL |
For Research Use Only. Not for use in diagnostic procedures.