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Invitrogen™ Ambion™ Proteinase K Powder DFS Item

General digestion of proteins

$145.95 - $548.10

Specifications

Fidelity (vs. Taq) Exonuclease Activity: 5' to 3'
Storage Requirements Store dry powder in desiccant at -20°C.
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

AM2542

DFS Item
 
invitrogen™
AM2542
100mg Each for $145.95

AM2544

DFS Item
 
invitrogen™
AM2544
500mg Each for $548.10
Description & Specifications

Specifications

Fidelity (vs. Taq) Exonuclease Activity: 5' to 3'
Storage Requirements Store dry powder in desiccant at -20°C.

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

  • Exonuclease Activity: 5' to 3'
  • Protein Filter: P
  • Species: T. album
Proteinase K Remains Active:
  • Over a wide pH range (optimal activity between 6.5 and 9.5)
  • Under denaturing conditions (e.g., the presence of SDS or urea)
  • In the presence of metal chelating agents (e.g. EDTA)
  • At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications:
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and Quality Testing:
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit Definition:
One mAnson unit is described as that amount of enzyme that liberates 1μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), DNA and RNA Purification and Analysis, DNA Extraction, General RNA Purification Reagents and Accessories, General gDNA Purification Reagents and Accessories, Genomic DNA Purification, RNA Extraction, RNAi, Epigenetics and Non-Coding RNA Research, Total RNA Isolation

Shipping Condition: Dry Ice