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Invitrogen™ Ambion™ FirstChoice™ RLM-RACE Kit DFS Item

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

$711.90 - $728.70

Products
Catalog Number Mfr. No. Includes Price Quantity    

AM1700M

DFS Item
 
invitrogen™
AM1700M
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature and manual Each for $711.90

AM1700

DFS Item
 
invitrogen™
AM1700
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature. Each for $728.70
Description & Specifications

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

  • From RNA to PCR product in less than a day
  • Yields single, specific product from rare transcripts
  • Selects 5' and/or 3' ends of true messages
  • Efficient—
  • Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
  • Final product size: 7kb or less
  • Optimal reaction temperature: 42°C
  • Sample: RNA

  • Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
  • Improvement to basic RACE protocol
  • Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Shipping Condition: Dry ice