One-Step Reverse Transcription PCR Reagents

An array of products designed to facilitate rapid, convenient reverse trancription PCR processes by simultaneously performing reverse transcription and amplification steps.

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Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Format	1-step RT-PCR Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 13 kb
Reaction Format	Master Mix
Reagent Type	Reverse Transcription
Hot Start	Built-in Hot Start
Optimal Reaction Temperature	50°C to 65°C
Technique	1-step RT-PCR
Starting Material	RNA
PCR Method	1-step RT-PCR
Multiplex Capability	Yes
Detection Method	Gel Electrophoresis

Invitrogen™ SuperScript™ IV One-Step RT-PCR System

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript IV
Final Product Type	PCR Amplified cDNA
Quantity	Each
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum SuperFi
Format	Kit
Size (Final Product)	Up to 14 kb
Reaction Format	Master Mix
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	45°C to 60°C
Technique	1-step RT-PCR
Starting Material	RNA

Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

Pricing and Availability

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	PCR Amplified cDNA
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum Taq High Fidelity
Format	One-Step RT-PCR System
Size (Final Product)	Up to 10 kb
Reaction Format	Premixed Components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	50°C
Technique	1-Step RT-PCR
Starting Material	RNA

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	PCR Amplified cDNA
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum Taq DNA Polymerase
Format	One-Step RT-PCR System
Size (Final Product)	Up to 4.5 kb
Reaction Format	Premixed Components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	50°C
Technique	1-Step RT-PCR
Starting Material	RNA

One-Step Reverse Transcription PCR Reagents

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