PCR Equipment and Supplies
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
| Physical Form | Liquid |
|---|---|
| pH | 7 |
| Chemical Name or Material | Saline-Sodium Citrate |
| Grade | Molecular Biology |
| DNase | DNase free |
| Recommended Storage | RT |
| Concentration | 2.9 to 3.1 M |
Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Hot-start PCR |
| Polymerase | Platinum Taq DNA Polymerase |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | Taq DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Applied Biosystems™ SequalPrep™ Normalization Plate Kit, 96-well
SequalPrep™ Normalization Plate Kit, 96-well
Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit, Fast 96-well
TaqMan™ hPSC Scorecard™ Kit, Fast 96-well
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Tube |
| Product Type | 1-Step qPCR kit |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Product Line | Cells-to-CT, TaqMan |
| Detection Method | TaqMan |
Applied Biosystems™ MiniAmp™ Thermal Cycler
The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs.
| Block Configurations | 96-well Isothermal Block |
|---|---|
| User Interface | USB, Wi-Fi, Ethernet |
| Wattage | 500 W |
| Dimensions | 15.4 x 7.5 x 7.9 in. (39 x 19 x 20 cm) |
| Max. Ramp Rate | 3°C/sec. (Block), 2.2°C/sec. (Sample) |
| High-throughput Compatibility | High-throughput Compatible |
| Weight | 13 lb. (5.9 kg) |
| Thermal Range | 0°C to 100°C |
| Display Type | 5 in. Color TFT LCD Touchscreen |
| Product Type | Thermal Cycler |
| Capacity | 96-well, 0.2 mL |
| Reaction Speed | Fast, Standard |
| Voltage | 100/240 V |
| Thermal Accuracy | ±0.25°C (35°C to 99.9°C) |
Applied Biosystems™ TaqMan™ Advanced miRNA Assay
Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR.
Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)
TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
| Shipping Condition | Dry Ice |
|---|---|
| InhibitorToleranceSimple | High |
| For Use With (Application) | Microbial Detection,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Sensitivity | Down to 10 copies |
| Format | Tube |
| Passive Reference Dye | None |
| For Use With (Equipment) | QuantStudio 6 Pro or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier |
| Sample Type | DNA, RNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Product Line | TaqPath |
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix
TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
| Shipping Condition | Dry Ice |
|---|---|
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Gene Expression,Microbial Detection |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Format | Tube |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | Mustang Purple |
| For Use With (Equipment) | QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx |
| Sample Type | DNA, RNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Product Line | TaqPath |
Invitrogen™ Precision gRNA Synthesis Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.
Applied Biosystems™ TaqPath™ ProAmp™ Master Mix
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. |
| InhibitorToleranceSimple | Very High |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Multiplex Capability | 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Taq DNA Polymerase |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Applied Biosystems™ QuantStudio™ 3 Real-Time PCR System, 96-well, 0.2 mL, laptop
QuantStudio™ 3 Real-Time PCR System is designed for users who need an affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality.
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
| Shipping Condition | Wet Ice |
|---|---|
| InhibitorToleranceSimple | Medium |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Multiplex Capability | Single-plex |
| Detection Method | SYBR |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Dual-Lock Taq DNA Polymerase |
| Format | Tube |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | PowerUP, SYBR |
Applied Biosystems™ QuantStudio™ 7 Pro Real-Time PCR System, 384-well
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Applied Biosystems™ QuantStudio™ 7 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint.