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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)

This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).

3

Invitrogen™ Platinum™ PCR SuperMix High Fidelity  DFS Item

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Shipping Condition Wet or Dry Ice
Polymerase Platinum Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 6X
Size (Final Product) 15 kb or less
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Mixed
Concentration 1.1X
For Use With (Application) Hot-start PCR,High-fidelity PCR
4

Invitrogen™ AccuPrime™ Pfx DNA Polymerase  DFS Item

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

Shipping Condition Wet or Dry Ice
Polymerase AccuPrime Pfx DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 26X
Size (Final Product) 12 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
Detection Method Primer-probe
5

Invitrogen™ Platinum™ SuperFi II DNA Polymerase  DFS Item

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.

Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
6

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase  DFS Item

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
7

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2  DFS Item

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Polymerase AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
8

Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase  DFS Item

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.

Shipping Condition Wet or Dry Ice
Polymerase Tsp Genotyping Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 500 bp or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR
9

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2  DFS Item

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
10

Invitrogen™ Max Bacterial Enhancement Reagent  DFS Item

The Max Bacterial Enhancement Reagent is designed for use with TRIzol™ Reagent to improve the isolation of intact total RNA up to three-fold from Gram-positive and Gram-negative bacteria.

11

Thermo Scientific Chemicals SSC (20X), RNAse free

Physical Form Liquid
Chemical Name or Material SSC
Recommended Storage Ambient temperatures
Concentration 20X
13

MilliporeSigma™ Calbiochem™ EMPIGEN™ BB Detergent, 30% Solution

Can be used as detergent for solubilization of membrane components

14

Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit  DFS Item
GREENER_CHOICE

The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

Shipping Condition Room Temperature
Target DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage

The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer.

Store at room temperature. Guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Clean-Up Kit
Sample Type PCR Products
Isolation Technology ChargeSwitch™ Chemistry
For Use With (Application) Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line ChargeSwitch
15

Invitrogen™ RNA Control 250 with Manual  DFS Item

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

Content And Storage RNA Control and THE RNA Storage Solution should be stored at –20°C
Format Tube
Label or Dye Unconjugated
Product Line Ambion