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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,006)
Singleplex and Multiplex Bead Based Immunoassays (527)
ELISA Substrates and Reagents (77)

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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ Mouse IL-33 Uncoated ELISA Kit with Plates

ELISA

Target Species Mouse
Conjugate HRP
Gene Symbol IL1rl1, IL33
Total Assay Time 24 hr 30 min
Sample Volume Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 47 pg/mL
Storage Requirements 2-8°C
Assay Range 47-3000 pg/mL
For Use With (Application) ELISA
Test Time 1 hr 45 min
Accession Number P14719, Q8BVZ5
Regulatory Status RUO
Target IL-33
Kit Contents Capture Antibody: Pre-titrated, purified antibody,Detection Antibody: Pre-titrated, HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 17082, 77125
Sample Type Serum,Supernatant
Label or Dye Biotin
Synonym Interleukin-33
3

Invitrogen™ Human ErbB3 (HER3) ELISA Kit  DFS Item

ELISA

Protein Family Growth Factors & Receptors
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
Assay Sensitivity 4 pg/mL
Research Discipline Extracellular Matrix & Cell Adhesion
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Her-3 (ErbB3)
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 4 pg/mL to 2500 pg/mL
Interassay CV <12%
Gene Symbol ErbB-3,HER3,LCCS2,MDA-BF-1,c-erbB-3,c-erbB3,erbB3-S,p180-ErbB3,p45-sErbB3,p85-sErbB3
Storage Requirements 2°C to 8°C
Assay Range 3.43 to 2500 pg/mL
Accession Number P21860
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 2065
Sample Type Plasma,Serum,Supernatant
Synonym ErbB-3, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
4

Invitrogen™ Human IL-8 ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Target Species Human
Conjugate FAM™
Gene Symbol GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.01 pg/mL
Storage Requirements -20°C
Assay Range 0.0128 to 5000 pg/mL
For Use With (Application) Cytokine Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P10145
Regulatory Status RUO
Target IL-8
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
5
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Invitrogen™ Human SOX17 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol SOX17
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.03 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.03 to 25 ng/mL
Test Time 1 hrs 20 mins
Accession Number HMG transcription factor SOX17; SOX17; Sox-17; SRY (sex determining region Y)-box 17; SRY box 17; SRY-box 17; SRY-box containing gene 17; SRY-box transcription factor 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
Regulatory Status RUO
Target SOX17
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 64321
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
7

Invitrogen™ Human IL-31 ELISA Kit

ELISA

Conjugate HRP
Interassay CV 3.3%
Intraassay CV 5.7
Gene Symbol Il31
Total Assay Time 4 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 1.2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 7,8 to 500 pg/mL
Test Time 1 hrs 20 mins
Accession Number Q6EBC2
Regulatory Status RUO
Target IL-31
Kit Contents Pre-coated 96 well plate,Standard (2 vials),Sample diluent,Biotinylated Detection Antibody,Assay buffer Concentrate,Wash buffer concentrate,SAV-HRP,TMB,Stop solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 386653
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
8

Invitrogen™ Human E-selectin (Soluble) ELISA Kit

ELISA

Conjugate HRP
Interassay CV 3.3%
Intraassay CV 5.7
Gene Symbol SELE
Total Assay Time 4 hrs 30 mins
Sample Volume Plasma 20 μL, Supernatant 20 μL
Assay Sensitivity 0.017 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 1.6 to 50 ng/mL
Test Time 1 hrs 20 mins
Accession Number P16581
Regulatory Status RUO
Target E-selectin (CD62E)
Kit Contents Pre-coated 96 well plate,Standard (2 vials),Sample diluent,Biotinylated Detection Antibody,Assay buffer Concentrate,Wash buffer concentrate,SAV-HRP,TMB,Stop solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6401
Sample Type Plasma,Supernatant
Label or Dye HRP
9

Invitrogen™ Human GITR ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 8784
Interassay CV 8.1%
Intraassay CV 3.3
Assay Sensitivity 9.7 pg/ml
Assay Range 62.3 to 4,000 pg/mL
10

Invitrogen™ Human IL-2 ELISA Kit  DFS Item

ELISA

Target Species Human
Conjugate HRP
Interassay CV 5%
Intraassay CV 7%
Total Assay Time 3 hr 30 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 9.1 pg/mL
Storage Requirements 2-8°C
Assay Range 18.8-1200 pg/mL
For Use With (Application) ELISA
Test Time 1 hr 20 min
Accession Number P60568
Regulatory Status RUO
Target IL-2
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3558
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
Synonym Aldesleukin, T cell growth factor, TCGF
11

Invitrogen™ Human PD-L2 ELISA Kit  DFS Item

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 80380
Interassay CV 3.1%
Intraassay CV 4.5
Assay Sensitivity 3.65 pg/ml
Assay Range 39 to 2,500 pg/mL
13

Invitrogen™ Human SARS-CoV-2 Spike (Trimer) Ig Total ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV 5%
Intraassay CV 4.3
Total Assay Time 1 hrs 15 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Assay Sensitivity <62.5 U/mL
Storage Requirements 2°C to 8°C
Assay Range 62.5 to 4000 Units/mL
Test Time 30 mins
Accession Number Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV-2
Regulatory Status RUO
Target SARS-CoV-2 Spike (trimer) Protein
Kit Contents Pre-coated 96 well plate,High Control (standard),Calibrator (medium control),Low Control,Anti-Ig Total - HRP conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
Label or Dye HRP
14

Invitrogen™ Mouse GM-CSF Uncoated ELISA Kit

ELISA

Target Species Mouse
Conjugate HRP
Gene Symbol 9230117N10Rik,Il-33,Il1f11,NF-HEV
Total Assay Time 24 hrs 30 mins
Sample Volume Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 4 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 4 to 500 pg/mL
For Use With (Application) ELISA
Test Time 1 hrs 45 mins
Accession Number P01587
Regulatory Status RUO
Target GM-CSF
Kit Contents Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Streptavidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 12981
Sample Type Serum,Supernatant
Label or Dye HRP
Synonym Granulocyte-macrophage colony stimulating factor, Colony-stimulating factor, CSF12
15

Invitrogen™ Rat TNF alpha Uncoated ELISA Kit with Plates

ELISA

Target Species Rat
Conjugate HRP
Gene Symbol Tnf
Total Assay Time 24 hr 30 min
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity 32 pg/mL
Storage Requirements 2-8°C
Assay Range 32-2000 pg/mL
For Use With (Application) ELISA
Test Time 1 hr 45 min
Accession Number P16599
Regulatory Status RUO
Target TNF alpha
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 24835
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
Synonym Tumor necrosis factor alpha, tnf-a, tnf-alpha