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Invitrogen™ Human E-selectin (Soluble) Instant ELISA™ Kit

Catalog No. 5017962
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5017962 128 Tests
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Catalog No. 5017962 Supplier Invitrogen™ Supplier No. BMS205INST

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Description

ELISA

The Human Soluble Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1) (Hu E-selectin) ELISA quantitates Hu sE-Selectin in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sE-Selectin Principle of the method The Human sE-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Specifications

Accession Number P16581
Assay Range 1.56 to 50 ng/mL
Assay Sensitivity 0.33 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6401
Gene Symbol CD62E,ELAM,ELAM1,ESEL,LECAM2
Interassay CV 7.5%
Intraassay CV 6.9
Kit Contents Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
Label or Dye HRP
Quantity 128 Tests
Regulatory Status RUO
Sample Volume Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
Storage Requirements -20°C
Synonym ELAM1, endothelial adhesion molecule 1, CD62E
Target E-selectin (CD62E)
Test Time 15 mins
Total Assay Time 3 hrs 10 mins
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