
PCR Equipment and Supplies

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
pH | 7 |
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Physical Form | Liquid |
Chemical Name or Material | Saline-Sodium Citrate |
Recommended Storage | RT |
Concentration | 2.9 to 3.1 M |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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InhibitorToleranceSimple | Medium |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Multiplex Capability | Single-plex |
Detection Method | SYBR |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | PowerUP™, SYBR™ |
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Shipping Condition | Dry Ice |
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Format | Kit |
Reverse Transcriptase | MultiScribe |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 120 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Quantity | 1 Ea. |
Technique | Reverse Transcription |
Concentration | 50 U/μL |
Starting Material | RNA |
Thermo Scientific™ dNTP Mix (10 mM each)
Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | >99% |
Form | Liquid |
Label or Dye | Unlabeled |
Concentration | 10 mM |
Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
GC-Rich PCR Performance | High |
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Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM)
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
Shipping Condition | Room Temperature |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 60 X |
Product Type | Gene Expression Assay |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
Polymerase | Platinum Taq DNA Polymerase |
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GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Starting Material | DNA |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Quantity | Each |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits
The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | Medium |
No. of Reactions | 100 Reactions |
Product Type | Mycoplasma Detection |
Form | Liquid |
Sample Type | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
Reaction Speed | Standard |
Label or Dye | FAM |
For Use With (Application) | Microbial Detection |
PCR Method | Multiplex qPCR |
Target Organism Class | Mollicutes, mycoplasma |
Product Line | MycoSEQ™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Shipping Condition | Wet or Dry Ice |
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Polymerase | Platinum Taq DNA Polymerase High Fidelity |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 6X |
Size (Final Product) | 15 kb or less |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | Mixed |
Concentration | 1.1X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Anchored Oligo(dT)20 Primer
Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC).
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq High Fidelity |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 10 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | PCR Amplified cDNA |
Quantity | Each |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum SuperFi |
Format | Kit |
Size (Final Product) | Up to 14 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 45°C to 60°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq DNA Polymerase |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 4.5 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |