Western Blotting Reagents, Membranes, Supplies

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Thermo Scientific™ Blocker™ Casein

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, mini DFS Item

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Thermo Scientific™ Western Blotting Filter Paper, Extra Thick, 20 cm x 20 cm

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Thermo Scientific™ Western Blotting Filter Papers, 0.83 mm thick, 8 x 10.5 cm

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection DFS Item

Novel fluorogenic probe for measuring oxidative stress in live cells

Invitrogen™ CellROX™ Deep Red Reagent, for oxidative stress detection DFS Item

Optimized probe for detection of oxidative stress in cells

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Invitrogen™ Measure-IT™ Thiol Assay Kit DFS Item

Provides easy and accurate quantitation of thiol

Thermo Scientific™ PVDF Transfer Membrane, 0.45 µm, 26.5 cm x 3.75 m

Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.

Thermo Scientific™ Blocker™ BSA (10X) in PBS

Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods.

Invitrogen™ iBlot™ 2 Gel Transfer Device DFS Item

Performs Western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers

MilliporeSigma™ Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

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