Western Blotting Reagents, Membranes, Supplies

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Invitrogen™ Novex™ PVDF Pre-cut Blotting Membranes DFS Item

Pre-cut and pre-assembled—ready for protein blotting/transfer

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Promega™ Western Blue™ Stabilized Substrate for Alkaline Phosphatase DFS Item

A stable, ready-to-use substrate for Western blots and immunoscreening

GE Healthcare Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

Invitrogen™ Molecular Probes™ CellROX™ Deep Red Reagent DFS Item

Optimized probe for detection of oxidative stress in cells

Invitrogen™ Mini Gel Tank and Blot Module Set DFS Item

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

GE Healthcare Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. GE Healthcare Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.

GE Healthcare Whatman™ Minifold I Dot Blot System

Well manifolds for proteins and nucleic acids

Invitrogen™ Novex™ iBlot™ 2 Nitrocellulose Transfer Stacks DFS Item

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Thermo Scientific™ Pierce™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

Hygiena ALLER-Snap Rapid Allergen Prevention Test

Liquid reagent contained in bulb of device. Pre-moistened swab in plastic tube.

MilliporeSigma™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

Invitrogen™ Novex™ Nitrocellulose Pre-Cut Blotting Membranes DFS Item

Ideal for blotting proteins and nucleic acids

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