Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Acrylamide: Bis-Acrylamide 37.5:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Invitrogen™ Mini Gel Tank DFS Item

Compatible with a variety of Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Thermo Scientific™ Owl™ EasyCast™ B1A Mini Gel Electrophoresis Systems

Cast and run a gel in the buffer chamber and achieve flat, even banding patterns on a consistent basis with these B1A Mini Gel Electrophoresis systems.

Acrylamide: Bis-Acrylamide 29:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Microplate Sealing Tape

Clear, self-sticking sealing tape for standard microplates.

Invitrogen™ Novex™ NuPAGE™ Bis-Tris Protein Gels 4 to 12%, Mini, 1.0 mm, 10-well DFS Item

Separation Range: 15 to 260 kDa (MOPS Buffer), 3.5 to 160 kDa (MES Buffer); Separation Type: Denaturing (MES Running Buffer, MOPS Buffer); For Use With: Mini Gel Tank, XCell SureLock Mini

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Fisherbrand™ Horizontal Mini Gel Electrophoresis System

Designed for quick separation of DNA or nucleic acids in agarose mini-gels, the Fisherbrand Mini Horizontal Gel System is a complete, compact mini gel electrophoresis system for easy nucleic acid separation. The horizontal system comes with an attached power supply,  two casting stands, six gel trays and four combs — everything necessary for DNA or nucleic acid, horizontal electrophoresis.

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Invitrogen™ Novex™ SimplyBlue™SafeStain DFS Item

Less hazardous and does not require methanol or acetic acid fixatives or destains

Invitrogen™ Novex™ NP40 Cell Lysis Buffer DFS Item

Suitable for preparation of cell extracts

Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder

A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Eisco™ Low Voltage AC/DC Power Supply

Offers AC voltages of 1.5, 3, 6, 9, 12V and regulated continuously variable DC voltages.

Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladder

A 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use as size standards in gel electrophoresis and Western blotting.

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