Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ PageRuler™ Prestained 10-180kDa Protein Ladder

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Acrylamide: Bis-Acrylamide 29:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Invitrogen™ Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels, 1.0mm, 10 well DFS Item

Pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Acrylamide: Bis-Acrylamide 37.5:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Thermo Scientific™ Pierce™ Silver Stain Kit

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Thermo Scientific™ Owl™ EasyCast™ B1A Mini Gel Electrophoresis Systems

Cast and run a gel in the buffer chamber and achieve flat, even banding patterns on a consistent basis with these B1A Mini Gel Electrophoresis systems.

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Thermo Scientific™ 6X DNA Loading Dye

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 10-Well DFS Item

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions

Fisher BioReagents™ EZ-Run™ Prestained Rec Protein Ladder, Fisher BioReagents

Comprised of 10 recombinant proteins covalently coupled to a blue chromophore plus 10 and 72kDa reference bands tagged with green and orange dyes, respectively

Invitrogen™ Bolt™ 4-12% Bis-Tris Plus Gels, 12-Well DFS Item

Precast polyacrylamide gels designed for optimal separation of small- to medium-sized proteins under denaturing conditions

Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific™ Pierce™ Reagent Reservoirs

Rapidly dispense reagents with multi-channel pipettors using these sterile, sturdy and disposable reservoirs molded from high-impact polystyrene.

EMD Millipore™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Invitrogen™ Mini Gel Tank DFS Item

Compatible with a variety of Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Thermo Scientific™ Microplate Sealing Tape

Clear, self-sticking sealing tape for standard microplates.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

EMD Millipore™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

EMD Millipore™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

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