Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

Mixture of 10 blue-, orange- and green-stained proteins (10 to 180kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Invitrogen™ Bolt™ 4 to 12%, Bis-Tris, 1.0 mm Mini Protein Gel, 12-well DFS Item

Separation Range: 3.5 to 260 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini

MilliporeSigma™ Immobilon™-P PVDF Transfer Membranes

Optimized for binding proteins transferred from a variety of gel matrices. Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Fisherbrand™ Horizontal Mini Gel Electrophoresis System

Designed for quick separation of DNA or nucleic acids in agarose mini-gels, the Fisherbrand Mini Horizontal Gel System is a complete, compact mini gel electrophoresis system for easy nucleic acid separation. The horizontal system comes with an attached power supply,  two casting stands, six gel trays and four combs — everything necessary for DNA or nucleic acid, horizontal electrophoresis.

Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Thermo Scientific™ Blocker™ Casein

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ Sealing Tape for 96-Well Plates

Clear, self-sticking sealing tape for standard microplates.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Owl™ EasyCast™ B1A Mini Gel Electrophoresis Systems

Cast and run a gel in the buffer chamber and achieve flat, even banding patterns on a consistent basis with these B1A Mini Gel Electrophoresis systems.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Acrylamide: Bis-Acrylamide 29:1 (40% Solution/Electrophoresis), Fisher BioReagents

CAS: 79-06-1 Molecular Formula: C3H5NO Molecular Weight (g/mol): 71.079 InChI Key: HRPVXLWXLXDGHG-UHFFFAOYSA-N PubChem CID: 6579 ChEBI: CHEBI:28619 IUPAC Name: prop-2-enamide SMILES: C=CC(=O)N

Invitrogen™ Bolt™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well DFS Item

Separation Range: 3.5 to 260 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini

Invitrogen™ Mini Gel Tank DFS Item

Compatible with a variety of Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Invitrogen™ NP40 Cell Lysis Buffer DFS Item

Suitable for preparation of cell extracts

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X) DFS Item

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Thermo Scientific™ Owl™ D3-14 Wide Gel Electrophoresis System

Screen PCR products, plasmid preps, restriction mapping and cloning with the Thermo Scientific™ Owl™ D3-14 Wide Gel Electrophoresis System.

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