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  5. Invitrogen™ Human Angiopoietin 1 ELISA Kit

Invitrogen™ Human Angiopoietin 1 ELISA Kit

Numéro de catalogue. EHANGPT1
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96 Tests
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Numéro de catalogue. Quantité
EHANGPT1 96 Tests
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Numéro de catalogue. EHANGPT1 Fournisseur Invitrogen™ Code fournisseur EHANGPT1

Description

ELISA

The Human Angiopoietin 1 (Hu APO1) ELISA quantitates Hu APO1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APO1. Principle of the method The Human APO1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Spécifications

Numéro d'enregistrement Q15389
Plage de l'essai 74.07 to 18,000 pg/mL
Sensibilité d’essai 30 pg/mL
Plage d’étalonnage 30 pg/mL to 18000 pg/mL
Essai Solid-phase sandwich ELISA
Conjugué HRP
Type de produit ELISA
Famille de protéines Kinase & Inhibitors
Type d’échantillon Plasma, Serum, Supernatant
Spécificité This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Suffisant pour 96 Assays
Espèces cibles Human
À utiliser avec (application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement) Colorimetric Microplate Reader
ID du gène (Entrez) 284
Symbole de gène AGP1,AGPT,ANG1
CV intra-essai <12%, <10
Contenus des kits Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Étiquette ou colorant HRP
Quantité 96 Tests
Statut réglementaire RUO
Disciplines de recherche Angiogenesis
Volume d’échantillon Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Conditions de stockage 2°C to 8°C
Synonyme AGP1, AGPT, ANG1
Cible Angiopoietin 1
Durée du test 1 hrs 20 mins
Durée totale de l'essai 4 hrs 45 mins
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