Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

181 – 195 of 537 results

181

Thermo Scientific™ Bst1107I (BstZ17I) (10 U/μL)

Cut at GTA^TAC sites with Bst1107I (BstZ17I) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssNAI, BstZ17I).

Pricing and Availability

182

Thermo Scientific™ Van91I (PflMI) (10 U/μL)

Cut at CCANNNN^NTGG sites with Van91I (PflMI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccB7I, BasI, PflMI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Van91I (PflMI)
Methylation Sensitivity	Dcm Methylation-Sensitive,Not Dam Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

183

Thermo Scientific™ BclI (10 U/μL)

Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I).

Pricing and Availability
Specifications

Concentration	10 U/μL

184

Thermo Scientific™ Mph1103I (NsiI) (10 U/μL)

The Mph1103I (NsiI) restriction enzyme recognizes ATGCA^T sites and cuts best at 37°C in R buffer (Isoschizomers: EcoT22I, NsiI, Zsp2I).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Mph1103I (NsiI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

185

Thermo Scientific™ CpoI (RsrII) (10 U/μL)

The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	CpoI (RsrII)
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

186

Thermo Scientific™ MunI (MfeI) (10 U/μL)

The MunI (MfeI) restriction enzyme recognizes C^AATTG sites and cuts best at 37°C in G buffer (Isoschizomers: MfeI).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	MunI (MfeI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

187

Thermo Scientific™ Bsp143I (Sau3AI) (10 U/μL)

Cut at ^GATC sites with Bsp143I (Sau3AI), which performs best at 37°C in its own unique buffer (Isoschizomers: BfuCI, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIm).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Bsp143I (Sau3AI)
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Unique Buffer (10X Buffer Bsp143I (Sau3AI))
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

188

Thermo Scientific™ HinfI (10 U/μL)

The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Hinf I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

189

Thermo Scientific™ BspTI (AflII) (10 U/μL)

Cut at C^TTAAG sites with BspTI (AflII) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AflII, BfrI, Bst98I, BstAFI, MspCI, Vha464I).

Pricing and Availability

190

Thermo Scientific™ BseNI (BsrI) (10 U/μL)

Cut at ACTGG(1/-1)^ sites with BseNI (BsrI) restriction enzyme, which performs best at 65°C in B buffer (Isoschizomers: Bse1I, BsrI, BsrSI).

Pricing and Availability
Specifications

Concentration	10 U/μL

191

Thermo Scientific™ NheI (10 U/μL)

The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).

Pricing and Availability
Specifications

Concentration	10 U/μL

192

Thermo Scientific™ Tru1I (MseI) (10 U/μL)

Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Tru1I (MseI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	65°C
Concentration	10 U/μL

193

Thermo Scientific™ BshNI (BanI) (10 U/μL)

Cut at G^GYRCC sites with BshNI (BanI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AccB1I, BanI, BspT107I).

Pricing and Availability

194

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BpiI (BbsI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

195

Thermo Scientific™ BstXI (10 U/μL)

Cut at CCANNNNN^NTGG sites with BstXI restriction enzyme, which performs best at 55°C in O buffer.

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BstXI
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer O
Type IIS RE	No
Concentration	10 U/μL

Restriction Enzymes

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