Restriction Enzymes
Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.
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ER de type IIS
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Enzyme
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Température de réaction optimale
- (10)
- (558)
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Sensible à l’inactivation par la chaleur
- (88)
- (528)
- (1)
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1
| Tampon de stockage | 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Composants | 25 to 50% Water, >50% Glycerin |
| Type de produit | NotI |
| Site de coupe | GC.GGCCGC |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Forme | Liquid |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I |
2
| Tampon de stockage | 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Composants | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
| Type de produit | BglII |
| Site de coupe | A.GATCT |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Forme | Liquid |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII |
4
| Tampon de stockage | 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 30°C |
| Composants | 10X OPTIZYME™ Buffer 4 |
| Type de produit | SmaI |
| Site de coupe | CCC.GGG |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Forme | Liquid |
| À utiliser avec (application) | >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI |
5
| Tampon de stockage | 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Composants | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride |
| Type de produit | HindIII |
| Site de coupe | A.AGCTT |
| Concentration | 10 U/μL |
| Forme | Liquid |
| Contenu et stockage | Keep container tightly closed |
| À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII |
6
| Tampon de stockage | 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
|---|---|
| Température de l’incubateur | 37°C |
| Composants | >50% Water, 25 to 50% Glycerin |
| Type de produit | ClaI (Bsu15I) |
| Site de coupe | AT.CGAT |
| pH | 7.4 |
| Concentration | 10 U/μL |
| Forme | Liquid |
| Contenu et stockage | Keep container tightly closed |
13
Thermo Scientific™ Cfr10I (BsrFI) (10 U/μL)
Cut R^CCGGY sites with Cfr10I (BsrFI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Bse118I, BsrFI, BssAIm).
14
Thermo Scientific™ Eco57I (AcuI) (5 U/μL)
The Eco57I (AcuI) restriction enzyme recognizes CTGAAG(16/14)^ sites and cuts best at 37°C in G (+SAM) buffer (Isoschizomers: AcuI).
| ER de type IIS | Yes |
|---|---|
| Température de réaction optimale | 37°C |
| Catégorie de recherche | Scarless Cloning |
| Sensibilité à la méthylation | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Type de produit | Restriction Enzyme |
| Enzyme | Eco57I (AcuI) |
| Sensible à l’inactivation par la chaleur | Yes |
| Concentration | 5 U/μL |
| Tampon compatible | 10X Buffer G |
15
Thermo Scientific™ SmaI, HC (50 U/μL)
Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).