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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Invitrogen™ MultiScribe™ Reverse Transcriptase  DFS Item

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

3

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase  DFS Item

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq High Fidelity
Format One-Step RT-PCR System
Size (Final Product) Up to 10 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
4

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System  DFS Item

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

GC-Rich PCR Performance High
Format 1-step RT-PCR Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 13 kb
Reaction Format Master Mix
Reagent Type Reverse Transcription
Hot Start Built-in Hot Start
Optimal Reaction Temperature 50°C to 65°C
Technique 1-step RT-PCR
Starting Material RNA
PCR Method 1-step RT-PCR
Multiplex Capability Yes
Detection Method Gel Electrophoresis
5

Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase  DFS Item

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.

Shipping Condition Wet or Dry Ice
Polymerase Tsp Genotyping Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 500 bp or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR
6

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2  DFS Item

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
7

Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix  DFS Item

The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.

Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold DNA Polymerase
Size (Final Product) 1.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Fast PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
8

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase  DFS Item

Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.

Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
9

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix  DFS Item

Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
10

Invitrogen™ Platinum™ Taq DNA Polymerase, High Fidelity  DFS Item

Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 6X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
For Use With (Application) Hot-start PCR,High-fidelity PCR
Detection Method Primer-probe
11

Applied Biosystems™ Platinum™ Multiplex PCR Master Mix  DFS Item

The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 2.5 kb or less
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR,Multiplex PCR
Multiplex Capability Up to 20-plex
12

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix  DFS Item
GREENER_CHOICE

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
13

Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase  DFS Item

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

14

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase  DFS Item

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

Shipping Condition Wet or Dry Ice
Polymerase AccuPrime GC-Rich DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR
15

Applied Biosystems™ True Allele™ PCR Premix  DFS Item

Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.