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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (4,016)
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1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2
Promotions Available

Invitrogen™ Human NPTXR ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol NPTXR
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.4 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.410 to 100 ng/mL
Test Time 1 hrs 20 mins
Accession Number Neuronal pentraxin receptor; NPR; NPTXR
Regulatory Status RUO
Target NPTXR
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 23467
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
3

Invitrogen™ Mouse CD30L (TNFSF8) ELISA Kit  DFS Item

ELISA

Protein Family CD Proteins
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 0.6 pg/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CD153
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL- 9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP- 1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM- 1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 Assays
Calibration Range 0.5 pg/mL to 100 pg/mL
Interassay CV <12%
Gene Symbol CD153,CD30LG,Cd30l,Tnlg3a
Storage Requirements 2°C to 8°C
Assay Range 0.41 to 100 pg/mL
Accession Number P32972
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 21949
Sample Type Plasma,Serum,Supernatant
Synonym CD153, CD30LG, Cd30l
4

Invitrogen™ Mouse TREM1 ELISA Kit  DFS Item

ELISA

Protein Family Chemokine & Receptors
Target Species Mouse
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 0.25 ng/mL
Research Discipline Immunology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target TREM1
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Sufficient For 96 Assays
Calibration Range 0.25 ng/ml to 200 ng/ml
Interassay CV <12%
Gene Symbol Trem1
Storage Requirements 2°C to 8°C
Assay Range 0.274 to 200 ng/mL
Accession Number Q9JKE2
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 58217
Sample Type Plasma,Serum,Supernatant
5

Invitrogen™ Human ALKP ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol ALPL
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 2 mIU/mL
Storage Requirements 2°C to 8°C
Assay Range 2 to 500 mIU/mL
Test Time 1 hrs 20 mins
Accession Number Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-AP
Regulatory Status RUO
Target ALPL
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 249
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
6

Invitrogen™ Human Kallikrein 6 ELISA Kit  DFS Item

ELISA

Protein Family GCPR
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 0.6 ng/mL
Research Discipline Neurobiology
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target Kallikrein 6
Specificity This ELISA pair antibody detects human KLK6. Other species not determined.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 0.6 ng/mL to 150 ng/mL
Interassay CV <12%
Gene Symbol Bssp,Klk7,PRSS18,PRSS9,SP59,hK6
Storage Requirements 2°C to 8°C
Assay Range 0.614 to 150 ng/mL
Accession Number Q92876
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 5653
Sample Type Plasma,Serum,Supernatant
Synonym Bssp, Klk7, PRSS18, PRSS9, SP59, hK6
7

Invitrogen™ Human LTF/Lactoferrin ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol LTF
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 1.96 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 1.96 to 480 ng/mL
Test Time 1 hrs 20 mins
Accession Number Bovine Lactoferrin; CKRX; Csp82; epididymis luminal protein 110; GIG12; Growth-inhibiting protein 12; HEL110; HLF2; Kaliocin 1; Kaliocin1; Kaliocin-1; lactoferricin; Lactoferricin H; Lactoferricin-B; Lactoferricin-H; Lactoferrin; lactoferroxin; LactoferroxinA; Lactoferroxin-A; LactoferroxinB; Lactoferroxin-B; LactoferroxinC; Lactoferroxin-C; Lactotransferrin; LF; Lfcin H; Lfcin-B; LfcinH; Lfcin-H; Ltf; minisatellite 10 r detected by probe MMS10; MMS10R; Ms10r; N lobe; neutrophil lactoferrin; talalactoferrin
Regulatory Status RUO
Target Lactoferrin
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4057
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
9
Promotions Available

Invitrogen™ Ovine IP-10 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol CXCL10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 28 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 28 to 7,000 pg/mL
Test Time 1 hrs 20 mins
Accession Number 10 kDa interferon gamma-induced protein; 10 kDa interferon-gamma induced protein precursor; C Cmotif chemokine; C x C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; crg-2; CXC; CXC chemokine; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; CXCL10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; gamma-IP10; gIP-10; H-IP-10; IFI10; Inp10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; protein Mob-1; Scyb10; similar to Small inducible cytokine B10 precursor (CXCL10) (10 kDa interferon-gamma induced protein) (Gamma-IP10) (IP-10); small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10
Regulatory Status RUO
Target CXCL10 (IP-10)
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 442997
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
10

Invitrogen™ Allopregnanolone ELISA Kit

ELISA

Conjugate HRP
Interassay CV 8%
Intraassay CV 7
Total Assay Time 16 hrs 45 mins
Sample Volume Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
Assay Sensitivity 129.7 pg/mL
Storage Requirements -20°C
Assay Range 156.25 to 10,000 pg/mL
Test Time 45 mins
Accession Number 3 alpha,5 alpha-tetrahydroprogesterone; 3 alpha-hydroxy-5 alpha-pregnan-20-one; allopregnanolone; Brexanolone; C21H34O2; Zulresso
Regulatory Status RUO
Target Allopregnanolone
Kit Contents Anti-IgG coated 96 well plate,Standard,Assay Buffer Concentrate,Capture Antibody,HRP-conjugated Antigen,Wash Buffer Concentrate,TMB Substrate,Stop Solution,Plate Sealer
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
11
Promotions Available

Invitrogen™ Human sFRP-3 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol FRZB
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 190 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 190 to 12,000 pg/mL
Test Time 1 hrs 20 mins
Accession Number Fiz; Fre; FREOS1; frezzled; Fritz; frizzled homolog-related; frizzled-related protein; frizzled-related protein 1; FRP; FRP-3; FRZB; FRZB1; FrzB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; OS1; Secreted frizzled-related protein 3; secreted frizzled-related sequence protein 3; Sfrp3; sFRP-3; SFRP3frezzled; SRFP3
Regulatory Status RUO
Target FRZB
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 2487
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
12

Invitrogen™ Mouse IgG2b Uncoated ELISA Kit

ELISA

Target Species Mouse
Conjugate HRP
Total Assay Time 24 hr 30 min
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
Assay Sensitivity 3.90 ng/mL
Storage Requirements 2-8°C
Assay Range 3.90-250 ng/mL
For Use With (Application) ELISA
Test Time 1 hr 45 min
Regulatory Status RUO
Target Mouse IgG2b
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym Immunoglobulin G2b
13
Promotions Available

Invitrogen™ Human Semaphorin-7A ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol SEMA7A
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.16 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.16 to 40 ng/mL
Test Time 1 hrs 20 mins
Accession Number 2900057C09Rik; Cd108; CDw108; H-Sema K1; H-SEMA-K1; H-Sema-L; JMH; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; john-Milton-Hargen human blood group Ag; MGC126692; MGC126696; M-Sema-L; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7 A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7 A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7 A; sema K1; Sema L; Sema7a; SEMAK1; Semal; semaphorin 7 A (John Milton Hagen blood group); semaphorin 7 A, GPI membrane anchor; semaphorin 7 A, GPI membrane anchor (John Milton Hagen blood group); Semaphorin K1; semaphorin-7 A; Semaphorin-K1; semaphorin-L; Semk1
Regulatory Status RUO
Target SEMA7A
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 8482
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
14
Promotions Available

Invitrogen™ Human Serpin A3/Alpha-1-Antichymotrypsin ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol SERPINA3
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.14 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.137 to 100 ng/mL
Test Time 1 hrs 20 mins
Accession Number AACT; ACT; Alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin His-Pro-less; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; growth-inhibiting protein 24; growth-inhibiting protein 25; MGC88254; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin A3; serpin family A member 3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; SERPINA3
Regulatory Status RUO
Target SERPINA3
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 12
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
15
Promotions Available

Invitrogen™ Human Serpin B3/SCCA1 ELISA Kit  DFS Item

ELISA

Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol SERPINB3
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 0.15 ng/mL
Storage Requirements 2°C to 8°C
Assay Range 0.156 to 10 ng/mL
Test Time 1 hrs 20 mins
Accession Number HsT1196; Protein T4-A; SCC; SCCA; SCCA1; SCCA-1; SCCA-PD; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serine protease inhibitor B3; serpin B3; serpin B4; serpin family B member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; serpin peptidase inhibitor, clade B (ovalbumin), member 4; Serpinb3; SERPINB4; spb3; Squamous cell carcinoma antigen 1; SSCA1; T4-A
Regulatory Status RUO
Target SERPINB3
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 6317
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP