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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide 
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide
Supplier: Invitrogen™ PV3793
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.Specifications
| Z´-LYTE™ | |
| FRET | |
| Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| Methylcoumarin | |
| 800 x 20 μL Assays | |
| myosin light chain 2 (KKRPQRRYSNVF) | |
| 800 Reactions | |
| End Point | |
| Peptide-Based Substrate | |
| CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2 |
| Serine/Threonine Kinases | |
| Serine/Threonine Kinase Assay | |
| Biochemical phospho-specific activity (no antibody) | |
| Kinase Assay Kit | |
| 384-well plate | |
| 405⁄465, 535 | |
| JBC(2001) 276, p38956 | |
| Dry Ice | |
| ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4 |
For Research Use Only. Not for use in diagnostic procedures.