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Invitrogen™ Wheat Germ Agglutinin (WGA) DFS Item

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.

$239.00 - $1064.00

Specifications

For Use With (Application) Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
Species Wheat
Protein Tag None
Expression System Wheat germ
Protein Family Lectins
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Products 13
Catalog Number Mfr. No. Conjugate Price Quantity  
Catalog Number Mfr. No. Conjugate Price Quantity  
W11263 DFS Item
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Invitrogen™
W11263
Alexa Fluor 350
Each for $610.00
 
W7024 DFS Item
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Invitrogen™
W7024
Alexa Fluor 350, Oregon Green 488, Tetramethylrhodamine, Texas Red
Each for $499.00
 
W11261 DFS Item
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Invitrogen™
W11261
Alexa Fluor 488
Each for $566.00
 
W32464 DFS Item
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Invitrogen™
W32464
Alexa Fluor 555
Each for $560.00
 
W11262 DFS Item
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Invitrogen™
W11262
Alexa Fluor 594
Each for $566.00
 
W21404 DFS Item
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Invitrogen™
W21404
Alexa Fluor 633
Each for $560.00
 
W32466 DFS Item
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Invitrogen™
W32466
Alexa Fluor 647
Each for $572.00
 
W32465 DFS Item
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Invitrogen™
W32465
Alexa Fluor 680
Each for $612.00
 
W834 DFS Item
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Invitrogen™
W834
Fluorescein
Each for $474.00
 
W6748 DFS Item
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Invitrogen™
W6748
Oregon Green 488
Each for $459.00
 
Q12021MP DFS Item
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Invitrogen™
Q12021MP
Qdot 655
Each for $1,064.00
 
W849 DFS Item
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Invitrogen™
W849
Tetramethylrhodamine
Each for $479.00
 
W21405 DFS Item
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Invitrogen™
W21405
Texas Red-X
Each for $239.00
 
Description

Description

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates. These lectins can bind to carbohydrates and are available conjugated to Alexa Fluor™, Alexa Fluor™ Plus, and other fluorescent dyes. Fluorescent wheat germ agglutinin conjugates are valuable tools in molecular and cell biology research, enabling researchers to label the plasma membrane in fluorescence imaging and cell painting assays, and study and analyze glycosylation patterns and glycan-mediated processes in cells and tissues.

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) with Alexa Fluor, Alexa Fluor Plus, and other dyes. WGA is a cell impermeant stain that selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues, which are often found on cell membranes. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.

These fluorescent lectin conjugates can also be used to label fixed cells; however, to avoid labeling intracellular components, formaldehyde-fixed cells should not be permeabilized before labeling. Fluorescent WGA conjugates are used as plasma membrane stains along with other cellular markers in cell painting assays to provide a phenotypic readout of cell health or cytotoxicity. The Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate is included in the Image-iT Cell Painting Kit (Cat. Nos. I65000 and I65500).

WGA conjugates are also used as retrograde tracers for neuronal tracing experiments and have been shown to cross synapses. These fluorescent lectins are applicable in microbiology studies to label yeast bud scars, the cell membrane of gram-positive but not gram-negative bacteria, and chitin in fungal cell walls. In solution, WGA exists as a heterodimer with a molecular weight of approximately 38,000 Daltons and is normally cationic under physiological conditions. Our WGA conjugates have been used in variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), and a wide range of chemical, biochemical and immunological assays.

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates with options covering the entire wavelength range. The Wheat Germ Agglutinin Sampler Kit (Cat. No. W7024) includes introductory samples of four fluorescent WGAs: Alexa Fluor 350, Oregon Green 488, tetramethylrhodamine, and Texas Red-X conjugates. The red-fluorescent Alexa Fluor 594 wheat germ agglutinin (WGA) conjugate is also included in the Image-iT LIVE Plasma Membrane and Nuclear Labeling Kit (Cat. No. I34406). The Texas Red-X WGA conjugate can be purchased in the ViaGram Red+ Bacterial Gram Stain and Viability Kit (Cat. No. V7023) to differentiate gram-positive and gram-negative bacteria.

Wheat germ agglutinin (WGA) conjugates fluorescence excitation/emission
W11261 WGA, Alexa Fluor 488 conjugate: 495 nm/519 nm
W11263 WGA, Alexa Fluor 350 conjugate: 346 nm/442 nm
W32464 WGA, Alexa Fluor 555 conjugate: 555 nm/580 nm
W11262 WGA, Alexa Fluor 594 conjugate: 590 nm/617 nm
W21404 WGA, Alexa Fluor 633 conjugate: 632 nm/647 nm
W32466 WGA, Alexa Fluor 647 conjugate: 650 nm/665 nm
W32465 WGA, Alexa Fluor 680 conjugate: 679 nm/702 nm
W56132 WGA, Alexa Fluor Plus 405 conjugate: 408 nm/450 nm
W56133 WGA, Alexa Fluor Plus 568 conjugate: 562 nm/583 nm
W56134 WGA, Alexa Fluor Plus 770 conjugate: 770 nm/797 nm
W834 WGA, fluorescein conjugate: 494 nm/518 nm
W6748 WGA, Oregon Green 488 conjugate: 496 nm/524 nm
W849 WGA, tetramethylrhodamine conjugate: 555 nm/580 nm
W21405 WGA, Texas Red-X conjugate: 595 nm/615 nm

Specifications

Specifications

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
None
Lectins
Agglutinin
N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Wheat
Wheat germ
Heterodimer
Fluorescent lectins
SDS
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For Research Use Only. Not for use in diagnostic procedures.