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Thermo Scientific™ VitroEase™ Training Kit

Catalog No. PIA51363
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25 Preparations
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PIA51363 25 Preparations
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Catalog No. PIA51363 Supplier Thermo Scientific™ Supplier No. A51363
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Description

The Thermo Scientific™ VitroEase™ Cryo-EM Training Kit provides a complete solution that includes detailed instructions for the successful preparation of grids for cryo-electron microscopy single particle analysis.

  • The VitroEase™ Cryo-EM Training Kit has been designed to provide the necessary components to get started with cryo-EM analysis of protein samples
  • Sample preparation is a major bottleneck in cryo-electron microscopy (cryo-EM) single particle analysis (SPA)
  • Currently, sample optimization is often performed in a non-systematic way, extending optimization time over multiple days or even weeks
  • Kit includes a positive control protein, apoferritin, that has been designed specifically to meet the stringent needs for high protein purity, minimal protein aggregation, and intact protein structure
  • Commonly used accessories for grid preparation are also included such as tweezers for manipulation, grids, and grid boxes for storage
  • Detailed step-by-step instructions guide the user through the lengthy vitrification process
  • Together the control and components may be used as a comparison for success for new users or for quality control for expert users
  • Grids and accessories are provided by trusted sources

Specifications

Content And Storage Sufficient for 25 grid preparations.

Contains VitroEase Apoferritin Standard (Cat. No. A51362), tweezers, grids, and grid boxes.
Description VitroEase™ Cryo-EM Starter Kit
For Use With (Application) Cryo-EM SPA
For Use With (Equipment) Cryo-Electron Microscopes
Quantity 25 Preparations

Frequently Asked Questions (FAQs)

What are the shipping and storage conditions for the VitroEase Training Kit?

The VitroEase Training Kit comes with two modules that are shipped separately. The Apoferritin Standard is shipped on dry ice and should be stored at -80 degrees C. It is also sold separately as Cat. No. A51362. The accessories (grids, tweezers, and Cryo TEM Grid Box) are shipped and stored at room temperature.
Is VitroEase Apoferritin Standard suitable for high resolution cryo-EM (electron microscopy)?

VitroEase Apoferritin Standard is suitable for high resolution cryo-EM (electron microscopy) and we have imaged it at 1.8 angstroms using our Krios G4 Cryo-TEM instrument.
What is the origin species for VitroEase Apoferritin Standard?

VitroEase Apoferritin Standard is human apoferritin heavy chain expressed in E. coli.