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Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells 
Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)
Supplier: Invitrogen™ K805020
Alert:
For Research Use Only. Not for use in diagnostic procedures.
Includes:
Box 1:
- pCR XL-2-TOPO vector
- Salt Solution
- T3 Primer
- T7 Primer
- XL Control PCR Template
- XL Control PCR Primers
- Platinum SuperFi Green PCR Master Mix
- SuperFi GC Enhancer
- Nuclease-free water
Box 2:
- Gel Solubilization Buffer (L3)
- Binding Buffer (B2)
- Wash Buffer (W1)
- Elution Buffer (E1)
- PureLink Clean-up Spin Columns (in Wash Tubes)
- PureLink Recovery/Elution Tubes
Box 3:
- One Shot OmniMAX 2 T1R chemically competent E. coli
- S.O.C. Medium
- pUC 19 Control DNA
Description
The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).The pCR-XL-2-TOPO vector includes:
- ccdB gene for positive selection
- EcoR I site flanking the PCR product insertion site for easy excision of inserts
- Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
- T7 promoter/priming site for in vitro RNA transcription and sequencing
- T7, T3, and M13 forward and reverse primer sites for sequencing
The TOPO XL-2 Complete PCR Cloning Kit includes:
- TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
- Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
- PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in less than 30 minutes
- One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells—an improved chemically competent cell line, perfect for use in all cloning applications.
Specifications
| OmniMAX2 | |
| Chemically Competent E. coli | |
| Box 1: Store at -5 to -30°C. • pCR XL-2-TOPO vector • Salt Solution • T3 Primer • T7 Primer • XL Control PCR Template • XL Control PCR Primers • Platinum SuperFi Green PCR Master Mix • SuperFi GC Enhancer • Nuclease-free water Box 2: Store at room temperature. • Gel Solubilization Buffer (L3) • Binding Buffer (B2) • Wash Buffer (W1) • Elution Buffer (E1) • PureLink Clean-up Spin Columns (in Wash Tubes) • PureLink Recovery/Elution Tubes Box 3: • One Shot OmniMAX 2 T1R chemically competent E. coli • S.O.C. Medium • pUC 19 Control DNA Store competent cells and pUC19 at -65 to -85°C. |
|
| PCR Cloning | |
| TOPO | |
| T3, T7 | |
| Approved for shipment at Room Temperature or on Wet or Dry Ice |
| Ampicillin (AmpR), Kanamycin (KanR) | |
| Zero Blunt TOPO | |
| Kit | |
| 20 Reactions | |
| Cloning Kit | |
| 20 reactions | |
| pCR-XL-2-TOPO |
For Research Use Only. Not for use in diagnostic procedures.