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Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™2 T1R Chemically Competent E. coli DFS Item

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

$479.95 - $822.15

Specifications

Product Type Cloning Kit
Storage Requirements Approved for shipment at Room Temperature or on Wet or Dry Ice
Vector TOPO Cloning Vectors
View More Specs
Includes:  Box 1:
  • pCR™ XL-2-TOPO vector
  • Salt Solution
  • T3 Primer
  • T7 Primer
  • XL Control PCR Template
  • XL Control PCR Primers
  • Platinum™ SuperFi™ Green PCR Master Mix
  • SuperFi GC Enhancer
  • Nuclease-free water
Box 2:
  • Gel Solubilization Buffer (L3)
  • Binding Buffer (B2)
  • Wash Buffer (W1)
  • Elution Buffer (E1)
  • PureLink Clean-up Spin Columns (in Wash Tubes)
  • PureLink Recovery/Elution Tubes
Box 3:
  • One Shot OmniMAX 2 T1R chemically competent E. coli
  • S.O.C. Medium
  • pUC 19 Control DNA

 Alert

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. No. of Reactions Price Quantity    

K805010

DFS Item
 
invitrogen
LSK805010
10 Each for $479.95
null of 0 Each for $479.95

K805020

DFS Item
 
invitrogen
LSK805020
20 Each for $822.15
null of 0 Each for $822.15
Description & Specifications

Specifications

Product Type Cloning Kit
Storage Requirements Approved for shipment at Room Temperature or on Wet or Dry Ice
Vector TOPO Cloning Vectors

The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments.  Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).

The pCR-XL-2-TOPO vector includes:

  • ccdB gene for positive selection
  • EcoR I site flanking the PCR product insertion site for easy excision of inserts
  • Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
  • T7 promoter/priming site for in vitro RNA transcription and sequencing
  • T7, T3, and M13 forward and reverse primer sites for sequencing
 
The TOPO XL-2 Complete PCR Cloning Kit includes:
  • TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector 
  • Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
  • PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes
  • One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells—an improved chemically competent cell line, perfect for use in all cloning applications.