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Invitrogen™ TNF alpha Monoclonal Antibody (TN3-19.12), eFluor™ 660, eBioscience™
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Numéro de catalogue. 50742382
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Quantité:
100 μg
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Numéro de catalogue. Quantité
50742382 100 μg
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Numéro de catalogue. 50742382 Fournisseur Invitrogen™ Code fournisseur 50742382

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Description

Armenian Hamster Monoclonal Antibody

Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. Applications Reported: This TN3-19.12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This TN3-19.12 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splecnoytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Spécifications

Antigène TNF alpha
Applications Flow Cytometry
Classification Monoclonal
Clone TN3-19.12
Concentration 0.2 mg/mL
Conjugué eFluor 660
Formule PBS with 0.09% sodium azide; pH 7.2
Gène Tnf
Numéro d’acquisition du gène P06804, P16599
Alias du gène APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Symboles de gène Tnf
Espèces hôtes Armenian Hamster
Méthode de purification Affinity chromatography
Quantité 100 μg
Statut réglementaire RUO
Primaire ou secondaire Primary
ID du gène (Entrez) 21926, 24835
Espèces cibles Mouse, Rat
Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
Type de produit Antibody
Forme Liquid
Isotype IgG
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