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Thermo Scientific™ Pierce™ BSA Protein Digest, MS grade

Catalog No. PI88341
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PI88341 1 nmol
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Catalog No. PI88341 Supplier Thermo Scientific™ Supplier No. 88341
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Description

Calibrate and maintain quality control for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples using the carefully validated Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade.

The Thermo Scientific Pierce BSA Protein Digest Standard, LC-MS Grade, is a validated protein digest optimized for use as a quality control sample for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples.

  • Positive control sample - BSA digest optimized and validated as a quality control standard for mass spectrometry applications
  • Excellent sequence coverage - greater than 85% sequence coverage
  • Validated peptide quality - digestion procedure optimized for minimal missed cleavages and over-alkylation
  • Rigorously tested - high-quality, consistent BSA protein digest documented via lot-specific certificates of analysis
  • Stable - provided in a stable lyophilized format
The Pierce BSA Protein Digest Standard is produced using high quality bovine serum albumin (BSA) and mass spectrometry-grade trypsin. The BSA is fully reduced, alkylated (iodoacetamide), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard that is free of intact protein. The peptide digest standard provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest standard has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Protein Digest
Product Line Pierce
Content And Storage Store in freezer (-5 to -30°C).
Starting Material Peptides
Final Product Type Peptides
Detection Method Mass Spectrometry
Workflow Step Calibration
Quantity 1 nmol
Form Solid

Frequently Asked Questions (FAQs)

How do I verify the LC-MS system after installation of a new column for proteomic applications?

Several technical replicate injections of a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) should be used to equilibrate and compare the new column to reference chromatograms.

How do I assess my LC-MS system performance?

The best way to assess the performance of an entire LC-MS system is using a mass spectrometry calibration solution, peptide retention time standards (Pierce Peptide Retention Time Calibration Mixture (PRTC), Cat. No. 88321 and Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix), Cat. No. A40010), and a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) specific to your application. We recommend frequent assessment of LC-MS system performance on a routine basis to compare results over time.
Which standards and calibrants do you recommend using for assessing LC-MS system suitability?

Here are our recommendations:

- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment

For Research Use Only. Not for use in diagnostic procedures.