Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). The Pierce Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific™ KingFisher™ Instruments, for high-throughput workflows.
- Specific - highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation
- Convenient and fast - product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour
- Low non-specific binding - stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments
- Versatile - beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)
Pierce Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0) 50mM NaOH, or SDS-PAGE sample buffer, the protocol has been optimized for each of these conditions. Anti-c-Myc antibody can be used to detect c-Myc tagged protein by Western blot analysis.
|High-affinity mouse IgG1 monoclonal antibody covalently coupled to blocked superparamagnetic beads; supplied at 10mg/mL in PBS containing 0.05% Tween-20 and 0.05% NaN3|
|Binding at least 100μg GST-c-Myc (26kDa fusion protein)/mL of bead suspension|