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Thermo Scientific™ Pierce™ 6 Protein Digest, equimolar, LC-MS grade

Catalog No. PI88342
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100 pmol
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PI88342 100 pmol
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Catalog No. PI88342 Supplier Thermo Scientific™ Supplier No. 88342
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Calibrate and maintain quality control for mass spectrometry (MS) analysis of proteomic samples using the Thermo Scientific™ Pierce™ 6 Protein Digest Standard, Equimolar, LC-MS Grade, a highly validated equimolar protein digest mixture.

The Thermo Scientific Pierce 6 Protein Digest Standard, Equimolar, LC-MS Grade, is a highly validated equimolar protein digest mixture for use as a quality control sample for mass spectrometry (MS) analysis of proteomic samples.

  • Positive control sample - optimized and validated as a quality control standard for mass spectrometry applications
  • Excellent sequence coverage - on average, greater than 70% sequence coverage of the 6-protein mixture
  • Validated peptide quality - digestion procedure optimized for minimal missed cleavages and over-alkylation
  • Rigorously tested - high-quality, consistent, 6-protein digest guaranteed and documented via lot-specific certificates of analysis
  • Stable - provided in a stable lyophilized format
The Pierce 6 Protein Digest Standard contains an equimolar mixture of highly pure bovine cytochrome c, lysozyme, alcohol dehydrogenase, bovine serum albumin, apo-transferrin, and beta-galactosidase. The proteins are reduced, alkylated (iodoacetic acid), digested (MS-grade trypsin), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard free of intact protein. The peptide digest provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Protein Digest
Product Line Pierce
Content And Storage Store in freezer (-5 to -30°C).
Starting Material Peptides
Final Product Type Peptides
Detection Method Mass Spectrometry
Workflow Step Calibration
Quantity 100 pmol
Form Solid

For Research Use Only. Not for use in diagnostic procedures.