missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Mass Spectrometry Sample Prep
  5. PI88342

Thermo Scientific™ Pierce™ 6 Protein Digest, equimolar, LC-MS grade

Catalog No. PI88342
Change view
Click to view available options
Quantity:
100 pmol
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI88342 100 pmol
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI88342 Supplier Thermo Scientific™ Supplier No. 88342
Only null left

Description

Calibrate and maintain quality control for mass spectrometry (MS) analysis of proteomic samples using the Thermo Scientific™ Pierce™ 6 Protein Digest Standard, Equimolar, LC-MS Grade, a highly validated equimolar protein digest mixture.

The Thermo Scientific Pierce 6 Protein Digest Standard, Equimolar, LC-MS Grade, is a highly validated equimolar protein digest mixture for use as a quality control sample for mass spectrometry (MS) analysis of proteomic samples.

  • Positive control sample - optimized and validated as a quality control standard for mass spectrometry applications
  • Excellent sequence coverage - on average, greater than 70% sequence coverage of the 6-protein mixture
  • Validated peptide quality - digestion procedure optimized for minimal missed cleavages and over-alkylation
  • Rigorously tested - high-quality, consistent, 6-protein digest guaranteed and documented via lot-specific certificates of analysis
  • Stable - provided in a stable lyophilized format
The Pierce 6 Protein Digest Standard contains an equimolar mixture of highly pure bovine cytochrome c, lysozyme, alcohol dehydrogenase, bovine serum albumin, apo-transferrin, and beta-galactosidase. The proteins are reduced, alkylated (iodoacetic acid), digested (MS-grade trypsin), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard free of intact protein. The peptide digest provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Protein Digest
Product Line Pierce
Content And Storage Store in freezer (-5 to -30°C).
Starting Material Peptides
Final Product Type Peptides
Detection Method Mass Spectrometry
Workflow Step Calibration
Quantity 100 pmol
Form Solid

Frequently Asked Questions (FAQs)

What are the UniProt IDs for the proteins in the Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342) standard?

The UniProt IDs for the proteins in the Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342) standard, are:
    Serum Albumin, Bovine (Uniprot: P02769)
    Cytochrome C, Bovine (Uniprot: P62894)
    Lysozyme, Chicken (Uniprot: P00698)
     β-galactosidase, E. coli (Uniprot: P00722)
    Apo-Transferrin, Bovine (Uniprot: Q29443)
    Alcohol Dehydrogenase, S. cerevisiae (Uniprot: P00330) or Mass Spectrometry Support Center.
How do I verify the LC-MS system after installation of a new column for proteomic applications?

Several technical replicate injections of a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) should be used to equilibrate and compare the new column to reference chromatograms.

How do I assess my LC-MS system performance?

The best way to assess the performance of an entire LC-MS system is using a mass spectrometry calibration solution, peptide retention time standards (Pierce Peptide Retention Time Calibration Mixture (PRTC), Cat. No. 88321 and Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix), Cat. No. A40010), and a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) specific to your application. We recommend frequent assessment of LC-MS system performance on a routine basis to compare results over time.
I don't need a complex digest for my MALDI study, but I need something more than a single protein digest. What do you recommend?

We recommend using the moderately complex Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342).
Which standards and calibrants do you recommend using for assessing LC-MS system suitability?

Here are our recommendations:

- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment

For Research Use Only. Not for use in diagnostic procedures.