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Thermo Scientific™ Yellow Sample Buffer (40X)

Catalog No. FERR1381
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4 x 1.25 mL
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FERR1381 4 x 1.25 mL
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Catalog No. FERR1381 Supplier Thermo Scientific™ Supplier No. R1381
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Description

40X Yellow Sample Buffer is used to color and track the samples while pipetting and setting up qPCR reactions.

Thermo Scientific™ 40X Yellow Sample Buffer is used with Thermo Scientific™ Luminaris™ Color qPCR Master Mixes to color and track samples. When using the blue Luminaris Color qPCR Master Mix the qPCR reaction mix is blue before sample addition. After adding the sample the reaction mix turns green, making the overall dispensing of qPCR reaction components quick, easy and error-free. This is particularly important for users of opaque white reaction plates and tubes.

The Yellow Sample Buffer is an aqueous solution of inert yellow dye (pH 8.0). The buffer is provided as a 40X concentrate and used in 1X concentration in the final reaction. The yellow dye does not affect the specificity or sensitivity of qPCR assays. The absorption maximum of the yellow dye is at 413nm.

Thermo Scientific 40X Yellow Sample Buffer is used with Luminaris Color qPCR Master Mixes to color and track the samples. When using the blue Luminaris Color qPCR Master Mix the qPCR reaction mix is blue before sample addition. After adding the sample the reaction mix turns green, making the overall dispensing of qPCR reaction components quick, easy and error-free. This is particularly important for users of opaque white reaction plates and tubes.

The Yellow Sample Buffer is an aqueous solution of the inert yellow dye (pH 8.0). The buffer is provided as a 40X concentrate and used in 1X concentration in the final reaction (for detailed recommendations refer to the manual of qPCR). The yellow dye does not affect the specificity or sensitivity of qPCR assays. The absorption maximum of the yellow dye is at 413 nm.

Highlights

  • Colored sample buffer for easy tracking during pipetting.

Absorption maximum: The absorption maximum of the yellow dye is at 413 nm
Hazardous: No
Storage Condition: -20°C

Specifications

Product Line Luminaris
Quantity 4 x 1.25 mL
Includes Contains:
• 4 x 1.25 mL 40X Yellow Sample Buffer

Store at -20°C.

Frequently Asked Questions (FAQs)

Does the yellow dye from the Yellow Sample Buffer provided with Luminaris Color and DyNAmo Flash qPCR Master Mixes influence qPCR performance?

The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.

For Research Use Only. Not for use in diagnostic procedures.