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TE buffer, pH 8.0, RNAse free
CAS: 1185-53-1 | C4H12ClNO3 | 157.594 g/mol
Supplier: Thermo Scientific Chemicals J62745EQE
Description
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
ApplicationsTrizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer. TE buffer is often used to store DNA and RNA while protecting it from degradation. Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.
Solubility
Not miscible or difficult to mix in water.
Notes
200mM Tris-HCl with 20mM EDTA
Specifications
| TE buffer | |
| 1185-53-1 | |
| Liquid | |
| C4H12ClNO3 | |
| Not miscible or difficult to mix in water. | |
| C(C(CO)(CO)N)O.Cl | |
| 157.594 |
| RNAse free | |
| 8.0 | |
| 125 mL | |
| MFCD00236359 | |
| QKNYBSVHEMOAJP-UHFFFAOYSA-N | |
| 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride | |
| 93573 |
Chemical Identifiers
| 1185-53-1 | |
| 157.594 | |
| QKNYBSVHEMOAJP-UHFFFAOYSA-N | |
| 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| C4H12ClNO3 | |
| MFCD00236359 | |
| 93573 | |
| C(C(CO)(CO)N)O.Cl |
Safety and Handling
TSCA : Yes
Recommended Storage : Ambient temperatures
RUO – Research Use Only