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Applied Biosystems™ TaqMan™ SCID/SMA Assay
A multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control.
Specifications
| Content And Storage | • DNA Extract All Reagents Kit • TaqPath ProAmp Multiplex Master Mix • TaqMan SCID/SMA Assay Upon receipt, store at -20°C. After opening, store at 4°C. |
|---|---|
| Detection Method | Primer-probe |
| For Use With (Equipment) | QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode) |
| Product Line | TaqMan |
| Shipping Condition | Dry Ice |
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
A47927
|
Applied Biosystems™
A47927 |
4000 Reactions |
N/A
|
N/A | |||||
| Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance. | |||||||||
Description
- A multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control
- For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (A48566)
- Optimized for SCID and SMA
- Direct extraction from DBS samples for reduced hands-on time and cost
- Compatible with 96- or 384-reaction protocols
- High-quality results for 96 samples within 55 min using the fast cycling protocol
- Minimal liquid-dispensing steps for easy automation
Complete solution from DBS to result
- The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker
- It is optimized for high specificity to SMN1, eliminating cross-detection of SMN2
- From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates
- One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed
- A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye
Specifications
| • DNA Extract All Reagents Kit • TaqPath ProAmp Multiplex Master Mix • TaqMan SCID/SMA Assay Upon receipt, store at -20°C. After opening, store at 4°C. |
|
| QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode) | |
| Dry Ice |
| Primer-probe | |
| TaqMan | |
| DNA (Genomic), cDNA |
For Research Use Only. Not for use in diagnostic procedures.