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Applied Biosystems™ TaqMan™ SCID/SMA Assay DFS Item

A multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control.

Specifications

Content And Storage • DNA Extract All Reagents Kit
• TaqPath ProAmp Multiplex Master Mix
• TaqMan SCID/SMA Assay

Upon receipt, store at -20°C. After opening, store at 4°C.

Detection Method Primer-probe
For Use With (Equipment) QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Product Line TaqMan
Shipping Condition Dry Ice
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Products 1
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
A47927 DFS Item
View Documents
Applied Biosystems™
A47927
4000 Reactions N/A
N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description

Description

  • A multiplex real-time PCR assay that detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control
  • For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (A48566)
  • Optimized for SCID and SMA
  • Direct extraction from DBS samples for reduced hands-on time and cost
  • Compatible with 96- or 384-reaction protocols
  • High-quality results for 96 samples within 55 min using the fast cycling protocol
  • Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result

  • The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker
  • It is optimized for high specificity to SMN1, eliminating cross-detection of SMN2
  • From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates
  • One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed
  • A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye
Specifications

Specifications

• DNA Extract All Reagents Kit
• TaqPath ProAmp Multiplex Master Mix
• TaqMan SCID/SMA Assay

Upon receipt, store at -20°C. After opening, store at 4°C.

QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Dry Ice
Primer-probe
TaqMan
DNA (Genomic), cDNA
Videos
SDS
missing translation for 'documents'

missing translation for 'documents'

For Research Use Only. Not for use in diagnostic procedures.