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Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

Supplier: Applied Biosystems™ 4366596

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Catalog No. 4366596

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Description

A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.

Components of this kit are used with the RT primer provided with the TaqMan™ MicroRNA Assay to convert miRNA to cDNA.

Order Info

Shipping Conditions: Dry Ice

Specifications

Content And Storage	Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/μL), and MultiScribe™ RT enzyme (50U/μL) for 200 reactions. Store at -15 to -25°C.
GC-Rich PCR Performance	High
Reaction Speed	Fast
Optimal Reaction Temperature	42°C
Product Type	MicroRNA Reverse Transcription Kit
Reverse Transcriptase	MultiScribe™
Sufficient For	200 Reactions
Concentration	10X
Final Product Type	cDNA
Reaction Format	Separate Components
Size (Final Product)	200 bp or less

Detection Method	Primer-probe
PCR Method	2-step RT-qPCR
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Product Line	TaqMan™
Quantity	200 reactions
Shipping Condition	Dry Ice
For Use With (Application)	miRNA analysis
Fidelity (vs. Taq)	10 X
No. of Reactions	200 Reactions
Sample Type	RNA

Product Suggestions Documents

Documents

Scientific Resources

Posters

Poster: miRNA Expression Profiling in Brain Tumor by Real-Time PCR File size: 1.2 MB

Poster: microRNA Quantitation by Real-time PCR File size: 205.6 kB

Poster: TaqMan-based miRNA Profiles Classify Embryonic Stem and Differentiated Cells File size: 472.4 kB

Poster: Plant microRNA Quantitation by Real-time PCR File size: 559.2 kB

Brochures

Brochures & Specifications: TaqMan MicroRNA Assays and Arrays File size: 346.8 kB

Product Information

Manuals

User Guide: TaqMan Small RNA Assays Version: J File size: 403.3 kB

Protocol for Running Custom RT and Preamplification Pools on Custom TaqMan Array MicroRNA Cards Version: January 2013 (Rev. A) File size: 272 kB

Product Info Sheet: MegaPlex Primer Pools Version: F File size: 156.1 kB

Promotions

Thermo Fisher Scientific , Applied Biosystems , Invitrogen

Get Up to $3,000 of qPCR Products with Purchase

Buy up to $3,000 of qPCR products and get a matching amount of qPCR products at no additional cost.

Expires: 06/30/25 Redemption Only

Limited Time
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Thermo Fisher Scientific , Applied Biosystems , Invitrogen

Get Up to $3,000 of qPCR Products with Purchase

Buy up to $3,000 of qPCR products and get a matching amount of qPCR products at no additional cost.

Expires: 06/30/25

Limited Time
See Full Promo Details

For Research Use Only. Not for use in diagnostic procedures.