missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Research and Clinical Analyzers and Instruments
  4. Microarray Kits
  5. 4444909

TaqMan™ Array Rodent MicroRNA A+B Cards Set v3.0

Catalog No. 4444909
Encompass
Change view
Click to view available options
Quantity:
4 x 2 cards
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
4444909 4 x 2 cards
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 4444909 Supplier Thermo Fisher Scientific Supplier No. 4444909
Only null left

Specifications

Type Array Rodent MicroRNA A+B Cards Set
Format 384-well Microfluidic Card
Species Mouse, Rat
Content And Storage Contents: 384-well arrays and information CD. Storage: The TaqMan™ MicroRNA Arrays are shipped at ambient temperature. Upon arrival, store arrays at 2 to 8 ° and keep them protected from light.
Form Dried Down
For Use With (Equipment) AB 7900 HT 384-Well System
Includes 384-Well Arrays, Information CD
Label or Dye FAM
Product Line TaqMan
Quantity 4 x 2 cards
Reaction Speed Standard
Research Category Gene Regulation, Epigenetics
Show More Show Less

Frequently Asked Questions (FAQs)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.
Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.
Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.
Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.
Why is the baseline variable when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the dried-down assays on the card were reconstituted at different rates, causing a dip in the early cycles of the baseline. We recommend that you use the relative threshold algorithm (Crt), which can correct for a variable baseline. If the software specific to your instrument does not have the relative threshold algorithm use the Relative Quantification app on Thermo Fisher Connect.
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why do my technical replicates have poor precision (standard deviation >0.5 for assays with a Ct value <30)?

Please review the following possible causes and solutions:
- Bubbles in wells. Use proper pipetting techniques to avoid introducing air into the fill reservoirs. Consider omitting the leaking wells from analysis.
- Wells leaking due to improper card sealing. When sealing the card, use a slow and steady motion to push the carriage across the TaqMan Array Card Sealer. We recommend that you consider omitting the leaking wells from analysis.
IMPORTANT: Do not move the carriage back across the card.
- Not all of the positions in the card holder were filled before centrifuging. Ensure that all of the empty positions of the card holder are filled with blank balance cards before centrifuging.
- The cards were centrifuged using a non-verified centrifuge. We recommend that you use a Sorvall centrifuge to prepare cards. Please see the Resources section at thermofisher.com/taqmanarrays for a list of compatible centrifuges.
- The dried-down assays on the card were reconstituted at different rates, causing a dip in the early cycles of the baseline. We recommend that you analyze results with the relative threshold algorithm (Crt) instead of the baseline threshold algorithm (Ct).
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Card, why is there no amplification within or across one or more rows of the card?

Please review the following possible causes and solutions:
- Empty wells due to improper card sealing. When sealing the card, use a slow and steady motion to push the carriage across the TaqMan Array Card Sealer. Do not move the carriage back across the card.
- Empty wells due to misalignment of the TaqMan Array Card Sealer. If the TaqMan Array Card Sealer is misaligned, contact Support.
-PCR reaction mix improperly prepared. Ensure that all reaction components were added to the PCR reaction mix.
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Card, why is there no amplification in some wells?

Usually, empty wells are due to improper card sealing. When sealing the card, use a slow and steady motion to push the carriage across the TaqMan Array Card Sealer. Do not move the carriage back across the card.
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Card, why is there no amplification for portions of the card?

Most likely the card was misaligned in the block during the instrument run. Inspect the card for crushed or distorted feet. If there are damaged feet, contact Support.
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why is there noise in the amplification plots for portions of the card?

Most likely the card was misaligned in the block during the instrument run. Inspect the card for crushed or distorted feet. If there are damaged feet, contact Support.
When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why is there gradient signal across the card?

This gradient signal indicates that the card was in a diagonal position during centrifugation because not all of the positions in the card holder were filled. We recommend that you repeat the assay with a new card and ensure that all the positions in the centrifuge card holder are filled.

For Research Use Only. Not for use in diagnostic procedures.