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Applied Biosystems™ TaqMan™ β-Actin Detection Reagents

Catalog No. 401846
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401846 100 reactions
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Catalog No. 401846 Supplier Applied Biosystems™ Supplier No. 401846
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Description

TaqMan™ β-Actin Detection Reagents

Applied Biosystems™ TaqMan™ β-actin provides the necessary components for using β-actin as a normalization control in TaqMan™ real-time PCR applications employing human nucleic acid. Either DNA or RNA can be used as a target template.

  • Pre-designed primers and FAM-labeled TaqMan™ probe eliminate assay design

  • Rapid assay development guidelines can minimize optimization time

  • Separate components provide flexibility in assay set-up

    Notes on Use

    The TaqMan™ β-actin Detection Reagent kit can be used with TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

    Notice to Purchaser
    1. Disclaimer of License: These products may be useful in the Polymerase Chain Reaction (PCR) methods covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. However, no license under these patents to use the PCR process is conveyed expressly or by implication to the purchaser by the purchase of this product.

    TaqMan™ probes are covered by US Patent 5,723,591 and foreign counterparts and patents pending owned by PE Corporation (NY), and may be covered by US Patents 5,801,155 and 6,084,102 and foreign counterparts licensed to Applied Biosystems™

    Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

    • Specifications

    Content And Storage Control Template(s)
    Control Template Human Control DNA
    Format Tube
    PCR Method 1-step RT-qPCR
    Gene Symbol ACTB
    Internal Probe Modification TAMRA™ Quencher (3'), FAM (5')
    Label or Dye FAM
    Product Line TaqMan
    Product Type Real Time PCR Reagents
    Quantity 100 reactions
    Shipping Condition Dry Ice
    Species Human
    Sufficient For 100 Reactions
    Target β-actin
    No. of Reactions 100 Reactions
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    Frequently Asked Questions (FAQs)

    Can I use the TaqMan β-actin Detection Reagents (Cat. No. 401846) as an endogenous control for my gene expression studies?

    The TaqMan β-actin Detection Reagents were designed to amplify genomic DNA, and thus are not recommended for use as an endogenous control for gene expression studies. However, we do offer other TaqMan assay reagents that were specifically designed for that application. Two examples are: Human ACTB (Beta Actin) Endogenous Control, Cat. No. 4310881E (TAMRA probe) or Cat. No. 4326315E (MGB probe).

    There are many factors to consider when validating a specific endogenous control. For additional information on designing gene expression assays and controls, please refer to the following documents: "Protocol: TaqMan Human Endogenous Control Plate", and User Bulletin #2: "Relative Quantitation of Gene Expression". You can find copies of these documents on our website by entering the titles in the main Search field.

    Can TaqMan Beta-actin Detection Reagents, Cat. No. 401846, be used for both human and mouse genomic DNA?

    This particular kit was designed for human DNA. Our current Beta-actin Detection Reagents are designed for human targets only. Please check our online search tools for other Beta actin reagents for human (Hs), mouse (Mm) or rat (Rn) targets.
    Where can I find the TaqMan Actin Detection Reagents (P/N 401846) manual?

    Complete instructions for the TaqMan B-actin Detection Reagents can be found in the manual titled "TaqMan PCR Reagent Kit with Controls" on the product page.

    For Research Use Only. Not for use in diagnostic procedures.