T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity). Unlike T4 RNA Ligase 1 , T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5 phosphate and 3' OH for ligation. The enzyme can also ligate the 3' OH of RNA to the 5 phosphate of DNA in a double stranded structure.
