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Molecular Probes™ Streptavidin, Pacific Orange™ conjugate

Catalog No. 501121545
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501121545 1 mg
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Catalog No. 501121545 Supplier Molecular Probes™ Supplier No. S32365
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Description

Streptavidin, Pacific Orange™ conjugate

Pacific Orange™ streptavidin has absorption/emission maxima of ∼400/551 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

Specifications

Conjugate Pacific Orange
Detection Method Fluorescence
For Use With (Equipment) Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Quantity 1 mg
Content And Storage Contains 1 vial of streptavidin Pacific Orange™ conjugate.

Store in freezer (-5 to -30°C) and protect from light.
Excitation/Emission 400/551
Flow Cytometer Laser Lines 405
Shipping Condition Room Temperature
Form Solid
Product Line Pacific Orange
Product Type Streptavidin Conjugate (Fluorescent)
Label or Dye Pacific Orange
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Frequently Asked Questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

For Research Use Only. Not for use in diagnostic procedures.