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Invitrogen™ SiteClick™ R-PE Antibody Labeling Kit, 1 kit

Catalog No. S10467
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Catalog No. S10467 Supplier Invitrogen™ Supplier No. S10467
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Description

Achieve precise and reproducible antibody labeling with SiteClick R-PE Antibody Labeling Kit.

Achieve precise and reproducible antibody labeling with SiteClick R-PE Antibody Labeling Kit. This kit targets carbohydrate domains present on all IgG antibodies, regardless of isotype or host species, ensuring site-selective labeling. The labeled antibodies are suitable for flow cytometry, fluorescence imaging, and western blot detection.

Features of the SiteClick R-PE Antibody Labeling Kit include:

  • Complete kit—includes everything needed to label 100 μg of IgG antibody using a straightforward, step-by-step protocol
  • Site-specific labeling—SiteClick technology attaches labels to the heavy chains of IgG antibodies under mild conditions, preserving antigen-binding domains
  • Consistent results—the gentle, site-selective attachment to heavy-chain N-linked glycans ensures reproducibility across sequential labelings and different antibodies, without harsh reduction steps
  • Efficient protocol—easy-to-follow steps requiring minimal hands-on time
  • High-quality conjugates—produces highly efficient, site-specific, and reproducible antibody conjugates

Unlike traditional amine or thiol labeling, which can affect antigen binding domains or antibody structure, SiteClick labeling technology maintains the integrity of the antibody. This ensures that antigen binding sites remain available for target binding, providing consistent and reproducible labeling. The workflow utilizes copper-free click chemistry to covalently link the DIBO moiety with the azide-modified antibody, ensuring uniform labeling without the need for reaction optimization.

With the SiteClick R-PE Antibody Labeling Kit, you can confidently achieve consistent, high-quality labeling every time.

Specifications

Kit Contents Contains everything needed to azido modify and label 100 μg of antibody.
Product Type Labeling Kit
Quantity 1 kit
Content And Storage Store the entire kit at 2 to 6°C upon receipt
Excitation/Emission 496, 546, 565/578
Labeling Method SiteClick Labeling System
Labeling Scale 100 μg
Shipping Condition Wet Ice
Product Line SiteClick
Label or Dye R-PE (R-Phycoerythrin)
Color Red-Orange
Labeling Target IgG
Flow Cytometer Laser Lines Blue, Green, Yellow
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Frequently Asked Questions (FAQs)

I need to label my tissue section with two mouse primary antibodies. Do I have to make direct conjugates of the antibodies to use them?

Check the isotype of the two antibodies to see if they are different. If so, we offer isotype-specific goat anti-mouse secondary antibodies that can be used together with minimal cross-reactivity. If the two mouse antibodies are of the same isotype, then you would need to make direct conjugates, such as with our antibody labeling kits or APEX, and SiteClick kits.

For Research Use Only. Not for use in diagnostic procedures.