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Cytiva Sephacryl™ S-500 High Resolution Media
Description
Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.
- For separating polysaccharides, macromolecules with extended structures, and small plasmids
- Excellent purification over a wide molecular weight range
- High reproducibility due to long-term chemical and physical stability
- High flow rates and recoveries
- Well-suited to industrial-scale use
- Storage conditions: 20% ethanol; 4° to 30°C
- Available in a variety of selectivities and volumes
Chemical Stability (stable in all common buffers):
- 1M acetic acid, 8M urea
- 6M guanidine-HCl, 1% SDS
- 2M NaCl, 24% ethanol
- 30% propanol, 30% acetonitrile
- 0.5M NaOH (for cleaning)
- 3 to 11 (long-term and working)
- 2 to 13 (short-term)
- Negligible volume variation due to changes in pH or ionic strength
- Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
- Antimicrobial agent: 20% ethanol
Specifications
Specifications
| Quantity | 150 mL |
| Flow Rate | >125 cm/hr. |
| Particle Size | 25 to 75 μm |
| Bed Volume | 19 to 230L |
| Product Type | Sephacryl HR Media |
| Format | Solid Spherical Bead |
| Diameter (Metric) Inner | 40 to 140cm |
| For Use With (Application) | Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale |
| Height (English) | 5.9 in. |
| Height (Metric) | 15cm |
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