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Cytiva Sephacryl™ S-400 High Resolution Media

Catalog No. 45002052
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Quantity:
150mL
Catalog No. Quantity
45002052 150mL
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Catalog No. 45002052

Supplier: Cytiva 17060910

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Description

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

  • For separating polysaccharides, macromolecules with extended structures, and small plasmids
  • Excellent purification over a wide molecular weight range
  • High reproducibility due to long-term chemical and physical stability
  • High flow rates and recoveries
  • Well-suited to industrial-scale use
  • Storage conditions: 20% ethanol; 4° to 30°C
  • Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

  • 1M acetic acid, 8M urea
  • 6M guanidine-HCl, 1% SDS
  • 2M NaCl, 24% ethanol
  • 30% propanol, 30% acetonitrile
  • 0.5M NaOH (for cleaning)
pH Stability
  • 3 to 11 (long-term and working)
  • 2 to 13 (short-term)
Physical Stability:
  • Negligible volume variation due to changes in pH or ionic strength
  • Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
  • Antimicrobial agent: 20% ethanol

Specifications

Quantity 150mL
Flow Rate >150 cm/hr.
Particle Size 25 to 75 μm
Bed Volume 19 to 230L
Product Type Sephacryl HR Media
Format Solid Spherical Bead
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laboratory and industrial scale
Height (English) 5.9 in.
Height (Metric) 15cm
Max. Pressure 100kPa
pH Range 3 to 11
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Sample Type Polysaccharides, macromolecules and small plasmids
Content And Storage 4° to 30°C
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