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Cytiva Sephacryl™ S-300 High Resolution Media

Catalog No. 45002058
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750 mL
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45002058 750 mL
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Catalog No. 45002058 Supplier Cytiva Supplier No. 17059901
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Description

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

  • For purifying antibodies, and serum and mid-size proteins
  • Excellent purification over a wide molecular weight range
  • High reproducibility due to long-term chemical and physical stability
  • High flow rates and recoveries
  • Well-suited to industrial-scale use
  • Storage conditions: 20% ethanol; 4° to 30°C
  • Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

  • 1M acetic acid, 8M urea
  • 6M guanidine-HCl, 1% SDS
  • 2M NaCl, 24% ethanol
  • 30% propanol, 30% acetonitrile
  • 0.5M NaOH (for cleaning)
pH Stability
  • 3 to 11 (long-term and working)
  • 2 to 13 (short-term)
Physical Stability:
  • Negligible volume variation due to changes in pH or ionic strength
  • Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
  • Antimicrobial agent: 20% ethanol

Specifications

Quantity 750 mL
Flow Rate >150 cm/hr.
Particle Size 25 to 75 μm
Bed Volume 19 to 230L
Product Type Sephacryl HR Media
Format Solid Spherical Bead
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of antibodies, serum proteins and mid-size proteins by gel filtration at laboratory and industrial scale
Height (English) 5.9 in.
Height (Metric) 15cm
Max. Pressure 100kPa
pH Range 3 to 11
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Sample Type Antibodies, serum and mid-size proteins
Content And Storage 4° to 30°C
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