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Thermo Scientific™ Microdase Disks

Numéro de catalogue. R21132
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Quantity:
25 Disks/Vial
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Numéro de catalogue. Quantity
R21132 25 Disks/Vial
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Numéro de catalogue. R21132 Fournisseur Thermo Scientific™ Code fournisseur RE21132
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Description

Rapidly differentiate Staphylococcus from Micrococcus species by detecting oxidase enzyme within 2 minutes with this reagent-impregnated disk.

Differentiate Staphylococcus from Micrococcus by detecting the oxidase enzyme with Thermo Scientific™ Remel™ Microdase Disk.

In 1956, Kovacs1 described the oxidase method that can be used to differentiate Gram-negative bacilli. Faller and Schleifer2 modified Kovacs' oxidase reagent by using a tetramethyl-p-phenylenediamine (TMPD) in dimethyl sulfoxide (DMSO). Tarrand and Groschel3 further tested modified Kovacs and confirmed it to be far superior to Kovacs' oxidase reagent.

All micrococci have cytochrome c whereas most staphylococci (with a few exceptions) lack this type of cytochrome. The oxidase reagent confirms the presence of cytochrome c4 by exhibiting color change to blue or purple.

  • Ready to use: Reagent-impregnated disk. No further preparation required.
  • Easy to read: Visible color change that confirms positive test
    Positive test: Blue or purple color development within 2 minutes
    Negative test: No color change (white to grey color remains) within 2 minutes.

Each Microdase Disk is impregnated with tetramethyl-p-phenylenediamine (TMPD) in dimethyl sulfoxide (DMSO). The DMSO provides solubility and stability against auto-oxidation and enhances the permeability of TMPD into the bacterial cell. In presence of atmospheric oxygen, the oxidase enzyme reacts with TMPD and cytochrome c in the organism to form the colored compound indophenol.

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Spécifications

Content And Storage 2°C to 8°C
Description Microdase Discs to differentiate staphylococci from micrococci
CE Marker Yes
Detectable Analytes Oxidase Enzyme
Format Vial
Quantity 25 Disks/Vial