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  5. Thermo Scientific™ Indole Broth (4mL)

Thermo Scientific™ Indole Broth (4mL)

Numéro de catalogue. R061172
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20 x 4 mL
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Numéro de catalogue. Quantity
R061172 20 x 4 mL
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Numéro de catalogue. R061172 Fournisseur Thermo Scientific™ Code fournisseur RE061172
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Description

Indole Broth is used to determine indole production by members of the Enterobacteriaceae.

Determine the indole production by members of the Enterobacteriaceae using Thermo Scientific™ Remel™ Indole Broth. Indole production is an important characteristic in the identification of many species of bacteria, specifically enteric gram-negative rods. Indole Broth is recommended by the American Public Health Association (APHA) and in the Identification of Enterobacteriaceae for use in the differentiation of enteric gram-negative rods1,2.

Microorganisms that possess the enzyme tryptophanase have the ability to hydrolyze and deaminate tryptophan with the production of indole, pyruvic acid and ammonia3. Indole that splits from the tryptophan molecule on addition of the reagent ρ-dimethylaminobenzaldehyde results in a distinct red color that can be easily detected4.

  • Ready to use - Liquid media
  • Recommended by APHA in the Identification of Enterobacteriaceae for use in the differentiation of enteric gram-negative rods1,2.
  • Easy to interpret -
    • Positive test - Pink to red colored ring at the interface of the broth and the reagent
    • Negative test - No color development

Indole Broth contains casein peptone that supplies essential growth factors, trace elements, and is the source of tryptophan in this medium.. Sodium chloride maintains osmotic equilibrium. Certain gram-negative rods release indole after hydrolyzing tryptophan. After addition of dimethylaminobenzaldehyde, it combines with the indole and produces a distinct red color.

Specifications

Description Indole Broth (Tryptone Broth)
Format 15mm x 103mm Tube
Product Type Microbiology Media
Quantity 20 x 4 mL