Thermo Scientific™ Remel™ BactiDrop™ Voges-Proskauer B

Description

Determine the ability of gram-negative bacilli to produce acetylmethylcarbinol (acetoin) from glucose fermentation using Thermo Scientific™ Remel™ Bactidrop™ Voges-Proskauer B Test. In 1898, Voges and Proskauer reported production of acetylmethylcarbinol from glucose fermentation by certain bacteria when grown in an appropriate medium such as MR-VP Broth which is a modification of Clark and Lubs medium 1,2. Later Barret made the VP test more sensitive by using 40% Potassium Hydroxide (VP-B) to the medium for the detection of acetylmethylcarbinol production3.

The BactiDrop™ Voges-Proskauer test is based on the detection of acetylmethylcarbinol production which is a precursor to 2,3-butanediol from glucose metabolism.Ready to use: Convenience of a prepared reagent.Ease of differentiation: VP positive organisms produce a pink color development while VP negative organisms produce no color.Rapid detection: Within 15 minutes.Glucose is metabolized to pyruvic acid from which the neutral products acetoin and 2.3-butanediol are formed. One molecule of acetoin is formed by the decarboxylation of two molecules of pyruvic acid. In the presence of oxygen and alkali, acetoin and 2,3-butanediol are oxidized to diacetyl which is the reactant for the pink color produced in Voges-Proskauer test. 40% potassium hydroxide aids in the absorption of CO2 to hasten the oxidation of acetoin to diacetyl.Used in conjunction with VP-A, Part No. R21560. For use with MRVP Broth.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.