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  6. Invitrogen™ RELM alpha Monoclonal Antibody (DS8RELM), Brilliant Violet™ 421, eBioscience™

Invitrogen™ RELM alpha Monoclonal Antibody (DS8RELM), Brilliant Violet™ 421, eBioscience™

Numéro de catalogue. 404544180
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25 μg
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404544182 100 μg
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Catalog No. 404544180 Supplier Invitrogen™ Supplier No. 404544180

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Description

Rat Monoclonal Antibody

This DS8RELM monoclonal antibody recognizes mouse RELM alpha, also known as FIZZ1. This antibody was shown not to cross-react with mouse RELM gamma. Applications Tested: This DS8RELM antibody has been tested by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols™: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Blocking Buffers

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Fixation

  • Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100...

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

Specifications

Antigène RELM alpha
Applications Flow Cytometry
Classification Monoclonal
Clone DS8RELM
Concentration 0.2 mg/mL
Conjugué Brilliant Violet 421
Formule PBS with BSA and 0.09% sodium azide; pH 7.2
Gène Retnla
Numéro d’acquisition du gène Q9EP95
Alias du gène 1810019L16Rik; cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; Fizz1; Fizz-1; FIZZ2; found in inflammatory zone 1; Himf; HXCP2; Hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; Pmng1; RELMa; RELMalpha; RELM-alpha; RELMb; RELMbeta; RELM-beta; resistin like alpha; resistin-like alpha; Retnla; Xcp2
Symboles de gène Retnla
Espèces hôtes Rat
Immunogène Recombinant mouse RELM alpha protein.
Méthode de purification Affinity chromatography
Quantité 25 μg
Statut réglementaire RUO
Primaire ou secondaire Primary
ID du gène (Entrez) 57262
Espèces cibles Mouse
Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
Type de produit Antibody
Forme Liquid
Isotype IgG1 κ
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