Recombinant Protein;Human Her2 / ErbB2 Protein;100UG;Human Her2, His Tag (HE2-H5225) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # AAA75493).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 0.05 g/mL (100 L/well) can bind Trastuzumab with a linear range of 0.2-3 ng/mL (QC tested).;Herceptin(Trastuzumab) capturedonCM5chipviaanti-human IgG Fc antibodies surface, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
