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Invitrogen™ ReadyProbes™ Tissue Autofluorescence Quenching Kit

Catalog No. R37630
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R37630 1 Kit
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Catalog No. R37630 Supplier Invitrogen™ Supplier No. R37630
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Description

Minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin

  • The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin.
  • If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays.
  • The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.
  • The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio.
  • Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature.
  • This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.

Specifications

Quantity 1 Kit
Content And Storage A kit containing 5 mL each ready-to-use (1X) solutions of reagent A, B, and C in three clear HDPE bottles.
  • Store at 2–8°C.
    • When stored as directed, product can be used for 3 months after receipt.One kit is sufficient to tre
    Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
    Product Line ReadyProbes
    Product Type Blocking Reagent

    Frequently Asked Questions (FAQs)

    What is the volume for one drop from the ReadyProbes dropper bottles?

    The volume for one drop from the ReadyProbes dropper bottles is approximately 41 µL.

    Does the ReadyProbes Tissue Autofluorescence Quenching Kit quench all autofluorescence?

    No. The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes autofluorescence created by aldehyde fixation, red blood cell autofluorescence, and autofluorescence of collagen and elastin. It will not quench natural pigments or other autofluorescent entities.
    Will the ReadyProbes Tissue Autofluorescence Quenching Kit still work if I messed up the order of addition, i.e., if I mixed Component A and Component C (instead of Component B) and then added Component B?

    For the ReadyProbes Tissue Autofluorescence Quenching Kit to work, the components need to be mixed in the recommended order. Component A needs to be mixed with Component B, followed by mixing in Component C.

    With the ReadyProbes Tissue Autofluorescence Quenching Kit, once I mix Components A, B, and C together, can I store this mixture in the refrigerator or freezer for later use?

    Once the 3 components are mixed together, we recommend using this mixture within 1 hour. The mixture should not be stored beyond 1 hour.

    For Research Use Only. Not for use in diagnostic procedures.