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Invitrogen™ ReadyProbes™ Streptavidin/Biotin Blocking Solution (1X)

Catalog No. R37628
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R37628 1 kit
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Catalog No. R37628 Supplier Invitrogen™ Supplier No. R37628

Description

ReadyProbes Streptavidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and streptavidin binding sites present in many types of tissue, reducing background signal in IHC, ICC, IF, and ISH procedures.

ReadyProbes Streptavidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and streptavidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures. This two-component solution (a streptavidin-blocking solution and a biotin-blocking solution) is provided in a ready-to-use format—just add a few drops of the streptavidin component and incubate for 15 minutes, then wash and repeat with the biotin component. ReadyProbes Streptavidin/Biotin Blocking Solution is used after the blocking of cells/tissue with standard non-specific blocking solution (e.g., diluted serum), but before the primary antibody step.

Specifications

Product Type Blocking Solution
Quantity 1 kit
Product Line ReadyProbes
Content And Storage A kit containing 18 mL each ready-to-use (1 X) solutions of streptavidin (component A) and biotin (component B) in two clear LDPE dropper bottles.

Store (at 2°C to 8°C.

When stored as directed, product can be used for 6 months after receipt.

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Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

Frequently Asked Questions (FAQs)

What is the volume for one drop from the ReadyProbes dropper bottles?

The volume for one drop from the ReadyProbes dropper bottles is approximately 41 µL.

In the ReadyProbes Streptavidin/Biotin Blocking Solution (1X) protocol, why is there a step to add biotin to the sample?

The first step in this kit is to bind all endogenous biotinylated entities with an unlabeled streptavidin. The free D-biotin is added to bind to all biotin binding sites on the added streptavidin. The free biotin will be washed away before any other labels are added and all biotin binding sites will be occupied on the unlabeled streptavidin.
I am labeling cells with a biotinylated primary antibody, followed by a streptavidin dye conjugate, but I'm getting non-specific mitochondrial labeling, even with a streptavidin-only control. Why?

Mitochondria have endogenous biotin, which is being labeled by the streptavidin. You will need to block the endogenous biotin first, after you fix and permeabilize, but before you block for your primary antibody.
How is ReadyProbes Streptavidin/Biotin Blocking Solution (1X) different from other endogenous biotin blocking kits?

The only difference between the ReadyProbes Streptavidin/Biotin Blocking Solution (1X) and other endogenous biotin blocking kits is the use of streptavidin (as other kits use avidin) and the ease of use. In the ReadyProbes Streptavidin/Biotin Blocking Solution (1X), we provide the two components already pre-mixed and ready to use.

For Research Use Only. Not for use in diagnostic procedures.