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Invitrogen™ ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (30X)
Description
ReadyProbes Mouse-on-Mouse IgG Blocking Solution blocks endogenous binding when mouse IgG primary antibody is used to label mouse tissue. Most mouse tissues contain mouse IgG antibodies. When an anti-mouse IgG secondary antibody is used on mouse tissue, the antibody cannot distinguish between the endogenous mouse IgG antibodies and the mouse primary antibody used for target protein detection. ReadyProbes Mouse on Mouse IgG Blocking Solution reduces the binding of endogenous antibodies, resulting in increased specific detection in immunohistochemistry (IHC) and immunofluorescence (IF) applications. This reagent is compatible with both fluorescent and chromogenic IHC procedures.
ReadyProbes Mouse-on-Mouse IgG Blocking Solution is provided at a 30X concentration and is diluted to a 1X working concentration (2 drops in 2.5 mL of PBS or TBS). This solution is not used in place of a non-specific blocking solution (e.g., diluted serum), but is used after the tissue has already been blocked with a non-specific solution and before the primary antibody step. Simply add the 1X solution to the tissue and incubate for 30-60 minutes at room temperature.
Specifications
Specifications
| Product Type | Blocking Solution |
| Quantity | 1 mL |
| Product Line | ReadyProbes |
| Form | Liquid |
| Content And Storage | Provided as 1-mL solution at 30 x concentration in a LDPE dropper bottle. Store (at 2°C to 8°C When stored as directed, product can be used for 6 months after receipt .One bottle is sufficient to treat approximately 125-250 tissue sections. ) |
| Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
Frequently Asked Questions (FAQs)
The volume for one drop from the ReadyProbes dropper bottles is approximately 41 µL.
Due to some cross-reactivity of anti-mouse antibodies to rat antibodies, using ReadyProbes Mouse-on-Mouse IgG Blocking Solution (30X) on rat tissues may offer some level of blocking, but it may not be sufficient. For rat tissues, ideally you should use a rat-on-rat blocking.
No. These two blocking steps cannot be combined. Doing so would result in inadequate blocking for both non-specific entities and mouse antibodies.
Assuming that the cultured mouse cells are not expressing mouse antibodies, ReadyProbes Mouse-on-Mouse IgG Blocking Solution (30X) is not needed for cultured mouse cells.
For Research Use Only. Not for use in diagnostic procedures.