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Invitrogen™ ReadyProbes™ In Situ Hybridization (ISH) Blocking Solution (5X)

Catalog No. R37620
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100 mL
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R37620 100 mL
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Catalog No. R37620 Supplier Invitrogen™ Supplier No. R37620
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Description

Minimizes non-specific background staining in chromogenic in situ hybridization (CISH) or fluorescence in situ hybridization (FISH) procedures

  • ReadyProbes In Situ Hybridization (ISH) Blocking Solution minimizes non-specific background staining in chromogenic in situ hybridization (CISH) or fluorescence in situ hybridization (FISH) procedures
  • It is provided as a 5X solution that is diluted to 1X with distilled or deionized water
  • After the labeled DNA or RNA probe hybridization and wash steps, the sample is incubated with 1X Ready Probes ISH Blocking Solution for 30–60 minutes
  • 1X ReadyProbes ISH Blocking Solution can also be used as diluent for antibodies, avidin, streptavidin, or biotin during incubation steps.

Specifications

Content And Storage Provided as 100-mL solution at 5X concentration in amber HDPE bottle.

Store at 2–8°C.

When stored as directed, product can be used for 6 months after receipt.
Concentration 5X
Product Line ReadyProbes
Product Type In Situ Hybridization Blocking Solution
Quantity 100 mL
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

Frequently Asked Questions (FAQs)

What is the volume for one drop from the ReadyProbes dropper bottles?

The volume for one drop from the ReadyProbes dropper bottles is approximately 41 µL.

Can the ReadyProbes In Situ Hybridization (ISH) Blocking Solution (5X) be used with PrimeFlow and ViewRNA labeling?

We do not recommend using the ReadyProbes In Situ Hybridization (ISH) Blocking Solution (5X) with PrimeFlow and ViewRNA labeling as the PrimeFlow and ViewRNA kits utilize a specific blocking reagent optimized for these kits.

For Research Use Only. Not for use in diagnostic procedures.