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Invitrogen™ ReadyProbes™ Avidin/Biotin Blocking Solution (1X)

Catalog No. R37627
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R37627 1 Kit
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Catalog No. R37627 Supplier Invitrogen™ Supplier No. R37627
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Description

Blocks endogenous biotin, biotin receptors, and avidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures

  • ReadyProbes Avidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and avidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures.
  • This two-component solution (an avidin-blocking solution and a biotin-blocking solution) is provided in a ready-to-use format—just add a few drops of the avidin component and incubate for 15 minutes, then wash and repeat with the biotin component.
  • ReadyProbes Avidin/Biotin Blocking Solution is used after the blocking of cells/tissue with standard non-specific blocking solution (e.g., diluted serum), but before the primary antibody step.

Specifications

Product Type Avidin/Biotin Blocking Solution
Quantity 1 Kit
Product Line ReadyProbes
Content And Storage A kit containing 18 mL each ready-to-use (1X) solutions of avidin (component A) and biotin (component B) in two clear LDPE dropper bottles.

Store at 2–8°C.

When stored as directed, product can be used for 6 months after receipt.
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

For Research Use Only. Not for use in diagnostic procedures.