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Invitrogen™ ReadyProbes™ Avidin/Biotin Blocking Solution (1X)

Catalog No. R37627
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R37627 1 Kit
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Catalog No. R37627 Supplier Invitrogen™ Supplier No. R37627
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Description

Blocks endogenous biotin, biotin receptors, and avidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures

  • ReadyProbes Avidin/Biotin Blocking Solution blocks endogenous biotin, biotin receptors, and avidin binding sites present in many types of tissue, reducing background signal in immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH) procedures.
  • This two-component solution (an avidin-blocking solution and a biotin-blocking solution) is provided in a ready-to-use format—just add a few drops of the avidin component and incubate for 15 minutes, then wash and repeat with the biotin component.
  • ReadyProbes Avidin/Biotin Blocking Solution is used after the blocking of cells/tissue with standard non-specific blocking solution (e.g., diluted serum), but before the primary antibody step.

Specifications

Product Type Avidin/Biotin Blocking Solution
Quantity 1 Kit
Product Line ReadyProbes
Content And Storage A kit containing 18 mL each ready-to-use (1X) solutions of avidin (component A) and biotin (component B) in two clear LDPE dropper bottles.

Store at 2–8°C.

When stored as directed, product can be used for 6 months after receipt.
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice

Frequently Asked Questions (FAQs)

With the ReadyProbes Avidin/Biotin Blocking Solution (1X), why is there a step to add biotin to the sample?

The first step is to bind all endogenous biotinylated entities with an unlabeled avidin. The free D-biotin is then added to bind to all biotin binding sites on this added avidin. The free biotin will be washed away before any other labels are added and all biotin binding sites will be occupied on the unlabeled avidin.
I am labeling cells with a biotinylated primary antibody, followed by an avidin dye conjugate, but I'm getting non-specific mitochondrial labeling, even with an avidin-only control. Why is this?

Mitochondria have endogenous biotin, which is being labeled by the streptavidin. You will need to block the endogenous biotin first, after you fix and permeabilize, but before you block for your primary antibody.
How is ReadyProbes Avidin/Biotin Blocking Solution (1X) different from other endogenous biotin blocking kits?

The only difference between the ReadyProbes Avidin/Biotin Blocking Solution (1X) and other endogenous biotin blocking kits is the ease of use. In the ReadyProbes Avidin/Biotin Blocking Solution (1X), we provide the two components already pre-mixed and ready to use.

For Research Use Only. Not for use in diagnostic procedures.