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Invitrogen™ ReadyProbes™ 2.5% Normal Horse Serum (1X)
Ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments
Supplier: Invitrogen™ R37625
Description
- ReadyProbes 2.5% Normal Horse Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a horse or donkey secondary antibody is used.
- Incubating cells/tissue with diluted serum from the same or similar species as the host of the secondary antibody typically reduces non-specific binding.
- Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Horse Serum and incubate for 30–60 minutes at room temperature.
- Ready Probes 2.5% Normal Horse Serum can also be used to dilute primary and secondary antibodies.
- Ready Probes 2.5% Normal Horse Serum is provided as a ready-to-use reagent with no further dilution or preparation required.
- The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay.
- The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.
Specifications
| Provided as 50-mL solution at ready-to-use (1X) concentration in a clear plastic dropper bottle. Store at 2–8°C. When stored as directed, product can be used for 6 months after receipt. |
|
| Liquid | |
| 50 mL | |
| Blocking Reagent |
| Horse | |
| Horse Serum | |
| ReadyProbes | |
| Room Temperature or Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.