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Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™

Numéro de catalogue. 501128837
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100 μg
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Numéro de catalogue. Quantité
501128837 100 μg
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Numéro de catalogue. 501128837 Fournisseur Invitrogen™ Code fournisseur 18401582
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Description

Rat Monoclonal Secondary Antibody

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 μg/mL. For western blotting, this can be used at 1 μg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Spécifications

Antigène Mouse IgG1
Applications ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone M1-14D12
Concentration 0.5 mg/mL
Conjugué HRP
Formule PBS with 50% glycerol and no preservative; pH 7.2
Espèces hôtes Rat
Méthode de purification Affinity chromatography
Quantité 100 μg
Statut réglementaire RUO
Primaire ou secondaire Secondary
Espèces cibles Mouse
Contenu et stockage 4°C
Type de produit Secondary Antibody
Forme Liquid
Isotype IgG
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