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  6. Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 615, eBioscience™

Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 615, eBioscience™

Numéro de catalogue. 42401582
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100 μg
25 μg
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Numéro de catalogue. Quantité
42401582 100 μg
42401580 25 μg
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Numéro de catalogue. 42401582 Fournisseur Invitrogen™ Code fournisseur 42401582

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Description

Rat Monoclonal Secondary Antibody

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Spécifications

Antigène Mouse IgG1
Applications Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone M1-14D12
Concentration 0.2 mg/mL
Conjugué eFluor 615
Formule PBS with 0.09% sodium azide; pH 7.2
Espèces hôtes Rat
Méthode de purification Affinity chromatography
Quantité 100 μg
Statut réglementaire RUO
Primaire ou secondaire Secondary
Espèces cibles Mouse
Contenu et stockage 4°C, store in dark, DO NOT FREEZE!
Type de produit Secondary Antibody
Forme Liquid
Isotype IgG
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