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  6. Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 615, eBioscience™

Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 615, eBioscience™

Catalog No. 42401582
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42401580 25 μg
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Catalog No. 42401582 Supplier Invitrogen™ Supplier No. 42401582
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Description

Rat Monoclonal Secondary Antibody

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Mouse IgG1
Applications Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone M1-14D12
Concentration 0.2 mg/mL
Conjugate eFluor 615
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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